STOOL ANTIGEN (HPSA) TEST IN DETECTION OF HELICOBACTER PYLORI INFECTION AMONG ADULT DYSPEPTIC PATIENTS IN TRIPOLI, LIBYA
DOI:
https://doi.org/10.11603/ijmmr.2413-6077.2019.2.10334Keywords:
Helicobacter pylori, dyspepsia, enzyme immunoassay, stool antigen testAbstract
Background. Helicobacter pylori is the most common infection in the world. Relationship between H. pylori and dyspepsia was confirmed by many studies, it has been strongly associated with peptic ulcer disease and gastric cancer. In that respect, several invasive and noninvasive methods for the diagnosis of H. pylori infection were utilized.
Objective. The aim of the study was to evaluate the association between dyspepsia and the positivity of H. pylori stool antigen test, to compare this test with serological IgG test.
Methods. 125 adult patients were randomly selected from gastroenterology units of Mediterranean and Tajurah clinics in Tripoli. Stool samples were taken for detection of H. pylori antigen by enzyme immunoassay. Blood samples for detection of anti-H. pylori IgG antibodies were taken. Data were statistically analyzed using SPSS.
Results. 125 dyspeptic patients: 47 male and 78 female, aged 18-83 years old were examined. 80 patients were infected by H. pylori that was proved by a positive stool test, 88 had a positive IgG test. The prevalence was higher in the patients aged 28-47 years old. There was substantial relation to age, marital status and economic risk factors; there was no association between H. pylori and gender, sources of drinking water, living standards, smoking, family history of peptic ulcer, drug consumption, and blood groups.
Conclusions. Relatively high rates of detection by HpSA prove that stool testing might be a reliable, simple, inexpensive, and non-invasive alternative test ащк detectшщт of H. pylori, diagnosing active infection and confirming cure. However IgG test has a low sensitivity, specificity, and accuracy compare to the HpSA test. Thus it can be used for screening purposes.
References
Asaka M, Sepulveda AR, Sugiyama T, Graham DY. Gastric cancer. In: Helicobacter pylori: physiology and genetics 2001. ASM Press.
Longo D, Fauci A. Harrison's Gastroenterology and Hepatology, 2e. McGraw-Hill Education; 2013.
Marcus EA, Scott DR. Gastric colonization by H. pylori. In: Helicobacter pylori. 2016 (pp. 23-34). Springer, Singapore.
doi: 10.1007/978-981-287-706-2_2
Moran AP. 10 Lipopolysaccharides of Helicobacter pylori: Importance in Gastric Adaptation and Pathogenesis. Helicobacter Pylori in the 21st Century. 2010;17:190.
doi: 10.1079/9781845935948.0190
Alm RA, Noonan B. The Genome. In: Mobley HLT, Mendz GL, Hazell SL, editors. Helicobacter pylori: Physiology and Genetics. Washington (DC): ASM Press; 2001. Chapter 27
Kim JM. H. pylori Virulence Factors: Toxins (CagA, VacA, DupA, OipA, IceA). In: Helicobacter pylori 2016 (pp. 77-88). Springer, Singapore.
doi: 10.1007/978-981-287-706-2_5
Kim N. Prevalence and transmission routes of H. pylori. In: Helicobacter pylori 2016 (pp. 3-19). Springer, Singapore.
doi: 10.1007/978-981-287-706-2_1
Plonka M, Targosz A, Brzozowski T. Can Drinking Water Serve as a Potential Reservoir of Helicobacter pylori? Evidence for Water Contamination by Helicobacter pylori. In: Trends in Helicobacter pylori Infection 2014 Apr 3. IntechOpen.
doi: 10.5772/57568
Sutton P, Mitchell H, editors. Helicobacter pylori in the 21st Century. CABI; 2010.
doi: 10.1079/9781845935948.0000
Walker BR, Colledge NR. Davidson's principles and practice of medicine e-book: With STUDENT CONSULT Online Access. Elsevier Health Sciences; 2013 Dec 6. P. 872-8.
Kim SE. Functional Dyspepsia. In: Helicobacter pylori, 2016 (pp. 207-218). Springer, Singapore.
doi: 10.1007/978-981-287-706-2_18
Yang HR. Updates on the diagnosis of Helicobacter pylori infection in children: what are the differences between adults and children? Pediatric Gastroenterology, Hepatology & Nutrition. 2016 Jun 1;19(2):96-103.
doi: 10.5223/pghn.2016.19.2.96
Dore MP, Pes GM, Bassotti G, Usai-Satta P. Dyspepsia: when and how to test for Helicobacter pylori infection. Gastroenterology Research and Practice. 2016;2016.
doi: 10.1155/2016/8463614
El-Shabrawi M, El-Aziz NA, El-Adly TZ, Hassanin F, Eskander A, Abou-Zekri M, Mansour H, Meshaal S. Stool antigen detection versus 13C-urea breath test for non-invasive diagnosis of pediatric Helicobacter pylori infection in a limited resource setting. Archives of Medical Science: AMS. 2018 Jan;14(1):69-73.
doi: 10.5114/aoms.2016.61031
Iranikhah A, Ghadir MR, Sarkeshikian S, Saneian H, Heiari A, Mahvari M. Stool antigen tests for the detection of Helicobacter pylori in children. Iranian Journal of Pediatrics. 2013 Apr;23(2):138-42.
Calik Z, Karamese M, Acar O, Karamese SA, Dicle Y, Albayrak F, Can S, Guvendi B, Turgut A, Cicek M, Yazgi H. Investigation of Helicobacter pylori antigen in stool samples of patients with upper gastrointestinal complaints. Brazilian Journal of Microbiology. 2016 Mar;47(1):167-71.
doi: 10.1016/j.bjm.2015.11.022
Asaka M, Kato M, Takahashi SI, Fukuda Y, Sugiyama T, Ota H, Uemura N, Murakami K, Satoh K, Sugano K. Guidelines for the management of Helicobacter pylori infection in Japan: 2009 revised edition. Helicobacter. 2010 Feb;15(1):1-20.
doi: 10.1111/j.1523-5378.2009.00738.x
Kalach N, Gosset P, Dehecq E, Decoster A, Georgel AF, Spyckerelle C, Papadopoulos S, Dupont C, Raymond J. A one-step immune-chromatographic Helicobacter pylori stool antigen test for children was quick, consistent, reliable and specific. Acta Paediatrica. 2017 Dec;106(12):2025-30.
doi: 10.1111/apa.13968
Tameshkel FS, Niya MH, Kheyri Z, Azizi D, Roozafzai F, Khorrami S. The evaluation of diagnostic and predictive values of Helicobacter pylori stool antigen test in Iranian patients with dyspepsia. Iranian Journal of Pathology. 2018;13(1):3838-44.
doi: 10.30699/ijp.13.1.39
Zhou. X, Su. J, Xu. G& Zhang, G. Accuracy of stool antigen test for the diagnosis of Helicobacter pylori infection in children: a meta-analysis. Clinics and research in Hepatology and gastroenterology, Oct 2014; 38 (5), 629-38.
doi: 10.1016/j.clinre.2014.02.001
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