EFFICACY OF GRANULOCYTE COLONY-STIMULATING FACTOR AND ENTEROSORPTION IN MELPHALAN-INDUCED BONE MARROW SUPPRESSION IN GUERIN CARCINOMA GRAFTED RATS

  • O. O. Shevchuk I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE
  • I. M. Todor R.E. KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY, ONCOLOGY AND RADIOBIOLOGY OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE
  • N. Yu. Lukianova R.E. KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY, ONCOLOGY AND RADIOBIOLOGY OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE
  • N. K. Rodionova INSTITUTE FOR NUCLEAR RESEARCH OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE
  • V. G. Nikolaev R.E. KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY, ONCOLOGY AND RADIOBIOLOGY OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE
  • V. F. Chekhun R.E. KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY, ONCOLOGY AND RADIOBIOLOGY OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE
Keywords: melphalan, Guerin carcinoma, rats, granulocyte colony stimulating factor, enterosorption

Abstract

Background. Side effects of antineoplastic agents (especially leukopenia and neutropenia) could be the main limiting factors for efficient treatment.

Objective. The research is aimed at the study of myeloprotective capability of biosimilars of granulocyte colony stimulating factor (G-CSF) and granular carbon oral adsorbent C2 in melphalan-induced bone marrow suppression in Guerin carcinoma-grafted rats.

Methods. Melphalan at the dose of 5.5 mg/kg was used to promote bone marrow suppression in the Guerin carcinoma grafted rats. To fight myelosuppression, we used filgrastim and its analogue, designed and produced by IEPOR, a recombinant granulocyte colony-stimulating factor (r-GCSF). Carbon granulated enterosorbent C2 was used for enteral sorption therapy (bulk density γ=0.18 g/cm3, diameter of granules 0.15-0.25 mm, BET pore surface – 2162 m2/g). All rats were sacrificed on the 17th day after carcinoma cells inoculation or on the 8th day after Melphalan injection.

Results. Alkylating cytostatic agent caused severe leukopenia (by 95.7%), neutropenia (by 73.9%), and thrombocytopenia (by 84.9%) in the experimental rats. Mortality rate was 57%. Filgrastim and enterosorption with carbon oral adsorbent C2 increased the studied indices, but the most prominent results were observed when combination of both factors was used. Studied means did not affect the anti-tumor efficacy of Melphalan alone and in combination.

Conclusions. Our results are perspective for further investigation of the efficacy of the combination of carbon oral adsorbents and hematopoietic cytokines in cases of ameliorate anti-cancer chemotherapy side effects, and its implementation into clinics.

Author Biographies

O. O. Shevchuk, I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE

MD, Ph.D., Associate Professor, Department of Pharmacology and Clinical Pharmacology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine.

ORCID 0000-0003-2473-6381

I. M. Todor, R.E. KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY, ONCOLOGY AND RADIOBIOLOGY OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE

Ph.D., DSc, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine.

N. Yu. Lukianova, R.E. KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY, ONCOLOGY AND RADIOBIOLOGY OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE

MD, Ph.D., DSc., R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine.

N. K. Rodionova, INSTITUTE FOR NUCLEAR RESEARCH OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE

MD, Ph.D., Institute for Nuclear Research of the National Academy of Sciences of Ukraine, Kyiv.

V. G. Nikolaev, R.E. KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY, ONCOLOGY AND RADIOBIOLOGY OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE

MD, Ph.D., DSc, Professor, Corr. Member of the NAS of Ukraine, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv.

V. F. Chekhun, R.E. KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY, ONCOLOGY AND RADIOBIOLOGY OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE, KYIV, UKRAINE

MD, Ph.D., DSc, Professor, Academician of the NAS of Ukraine, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv. e-mail: chekhun@onconet.kiev.ua

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Published
2019-07-12
How to Cite
Shevchuk, O. O., Todor, I. M., Lukianova, N. Y., Rodionova, N. K., Nikolaev, V. G., & Chekhun, V. F. (2019). EFFICACY OF GRANULOCYTE COLONY-STIMULATING FACTOR AND ENTEROSORPTION IN MELPHALAN-INDUCED BONE MARROW SUPPRESSION IN GUERIN CARCINOMA GRAFTED RATS. International Journal of Medicine and Medical Research, 5(1), 66-74. https://doi.org/10.11603/ijmmr.2413-6077.2019.1.10307