ANALYSIS OF THE USE OF MODERN IN VIVO MICROSCOPY METHODS FOR THE DIAGNOSIS AND TREATMENT OF MALIGNANT NEOPLASMS
DOI:
https://doi.org/10.11603/mie.1996-1960.2023.1-2.13956Keywords:
intravital microscopy, malignant neoplasms, diagnosis, treatmentAbstract
Background. In vivo endoscopic optical microscopy provides a tool to assess tissue architecture and morphology similar to standard histological examination without the need for tissue removal. Real-time in vivo imaging has become an integral tool for the investigation and understanding of cellular processes in health and disease at single-cell resolution, has revolutionized visualization of tumor-microenvironment interactions in real time. At the same time, there are still many unresolved questions regarding the clear interpretation of the results obtained with the intravital microscopy, indications for the clinical application of each of the technologies, the formation of clinical protocols with the inclusion of appropriate methods for different nosology, modification of surgical instruments with the involvement of optical systems, etc.
Materials and methods. Theoretical analysis, generalization, and systematization of research results were carried out in accordance with PRISMA Group recommendations using such leading scientometric databases as PubMed, Medline, PILOTS Ovid EMBASE, Ovid Cochrane Central Register of Controlled Trials, Ovid Cochrane Database of Systematic Reviews, Ovid PsycINFO, Global Health Library CINAHL, and the Web of Science and Scopus, for the period from 2003 to 2023 by keywords: intravital microscopy, intravital multipho-ton microscopy, fluorescence confocal microscopy, photodynamic diagnostics, optical coherence tomography, confocal laser endomicroscopy, atomic force microscopy, computer image processing.
Results. In this article, we focus on optical imaging technologies in vivo that have the powerful potential to significantly improve the diagnosis and therapy of malignant tumors: fluorescence confocal microscopy, optical coherence tomography, light sheet microscopy, two-photon and high-resolution microscopy, atomic force microscopy, electron microscopy, etc. We investigated the technological principles, preclinical and clinical studies analyzing the sensitivity and specificity of the above methods in the diagnosis and treatment of various types of malignant neoplasms, methods of computer image processing, discussed the prospects for improving the above technologies, further prospects for the development of the latest optical devices for the diagnosis and treatment of malignant neoplasms. We also address the advantages and limitations of this high-resolution technologies.
Conclusions. Major technological advances are rapidly expanding the frontiers of intravital microscopy, which is likely to play an increasingly important role in preclinical, clinical cancer research, diagnosis and treatment of malignant neoplasms in the coming years.
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