Evaluating the hypoglycaemic, anti-inflammatory, and antioxidant effects of Hibiscus sabdariffa in alloxan-induced diabetic rats
DOI:
https://doi.org/10.63341/bmbr/1.2025.33Keywords:
oxidative stress biomarkers, pro-inflammatory cytokines, bioactive compounds, pancreatic β-cellsAbstract
Hibiscus sabdariffa is beneficial in treating diabetes mellitus. This study investigated the hypoglycaemic, antiinflammatory, and antioxidant effects of Hibiscus sabdariffa in alloxan-induced diabetic rats. Thirty Wistar rats were divided into six groups of five and acclimatised for two weeks before the experiment commenced. Group I: non-diabetic control; Group II: diabetic control; Group III: non-diabetic with 200 mg/kg of Hibiscus sabdariffa; Group IV: non-diabetes with 300 mg/kg of Hibiscus sabdariffa; Group V: diabetic with 200 mg/kg of Hibiscus sabdariffa; Group VI: diabetic with 300 mg/kg of Hibiscus sabdariffa. The rats received a single intraperitoneal injection of alloxan (150 mg/kg of body weight), and diabetic rats were treated with Hibiscus sabdariffa for 21 days. Fasting blood glucose levels, insulin levels, superoxide dismutase, catalase, malondialdehyde, interleukin-6, and tumour necrosis factor-alpha were measured, and organ and blood samples were collected. The results were analysed using analysis of variance with p<0.05 considered significant, and data were visualised using GraphPad. This study demonstrated that Hibiscus sabdariffa exerts significant effects on diabetic
parameters, pro-inflammatory cytokines, and antioxidant enzymes. Daily oral treatment for 21 days lowered fasting blood glucose, interleukin-6, tumour necrosis factor-alpha, and malondialdehyde levels. It also enhanced insulin production, superoxide di smutase, and catalase activity in the skeletal muscle, liver, pancreas, and kidney. It can be concluded that
Received: 14.08.2024 | Revised: 27.11.2024 | Accepted: 25.02.2025
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