Mouthwash as a factor in controlling the formation of soft dental plaque in patients with orthodontic treatment
DOI:
https://doi.org/10.61751/bmbr/2.2024.38Keywords:
microbiocenosis, microorganisms, oral cavity, gingivitis, dental braceAbstract
Patients with dental braces are more likely to experience rapid accumulation of dental plaque and gum
inflammation, therefore, antimicrobial mouthwashes that enhance daily oral hygiene can effectively remove and control
bacterial plaque, improve gum health, and are safe preventive agents without side effects. The aim of the study was to
evaluate the quantitative and qualitative composition of the soft dental plaque microbiocenosis under the influence of an
alcohol-free oral hygiene product in patients undergoing orthodontic treatment. The study included patients undergoing
orthodontic treatment. Samples were taken from the surface teeth of the maxilla. The obtained swabs from the surface
of the upper molars were examined using a bacteriological method with subsequent identification: microscopic, cultural,
and biochemical. Comparison of the effectiveness of an alcohol-free mouthwash in terms of changes in the quantitative
and qualitative composition of microorganisms and the condition of periodontal tissues in patients undergoing stationary
orthodontic therapy carried out after three months of use. The study revealed gram-positive and gram-negative bacteria
and Candida fungi with a subsequent insignificant decrease in gram-negative bacteria (p = 0.999) after using the
mouthwash. The results obtained showed changes in the taxonomic composition, namely, a decrease in the number of
bacterial genera from 11 to 9. In addition to the mentioned results, changes were found at the population level of the soft
dental plaque microbiota in patients who used the alcohol-free mouthwash for three months, namely, a decrease in the
number of Streptococcus spp. α p(x ≤ T) = 0.9958, Propionibacterium spp. p(x ≤ T) = 0.9837, while changes in Streptococcus
spp. β and Streptococcus spp. γ were minimal. A comparison of the population level of microorganisms before and after the
use of mouthwash revealed significant differences (p < 0.05). Determination of the gingival index demonstrated a slight
improvement in the periodontal status of the examined subjects. The conducted study is of great importance for the
development of new strategies for the prevention and treatment of oral cavity diseases
Received: 08.02.2024 | Revised: 22.04.2024 | Accepted: 28.05.2024
References
Kamran MA, Alnazeh AA, Almoammar S, Almagbol M, Baig EA, Alrwuili MR, et al. Effect of plant-based mouthwash (Morinda citrifolia and Ocimum sanctum) on TNF-α, IL-α, IL-β, IL-2, and IL-6 in gingival crevicular fluid and plaque scores of patients undergoing fixed orthodontic treatment. Medicina. 2023;59(11):1968. DOI: 10.3390/medicina59111968
Goyal N, Shamanna PU, Varughese ST, Abraham R, Antony B, Emmatty R, et al. Effects of amine fluoride and probiotic mouthwash on levels of Porphyromonas gingivalis in orthodontic patients: A randomized controlled trial. J Indian Soc Periodontol. 2019;23(4):339–44. DOI: 10.4103/jisp.jisp_551_18
Hamdoon SM, AlSamak S, Ahmed MK, Gasgoos S. Evaluation of biofilm formation on different clear orthodontic retainer materials. J Orthod Sci. 2022;11:34. DOI: 10.4103/jos.jos_7_22
Mahjoub DT, AlJabri RK, Bifari NE, Najjar RS. Oral hygiene awareness and practice in orthodontic patients in Makkah city: A cross-sectional study. J Orthod Sci. 2023;12(1):32. DOI: 10.4103/jos.jos_115_22
Shahi AK, Kumar P, Shetty D, Jain A, Sharma P, Raza M. Effect of antimicrobial agents on the oral microflora in patients undergoing fixed orthodontic therapy – An ex vivo comparative analysis. J Orthod Sci. 2021;10(1):12. DOI: 10.4103/jos.JOS_46_20
Manashchuk N, Chorny N, Boytsanyuk S, Zaliznyak M, Chorny A. A modern look at the use of rinses for the oral cavity. Clin Dent. 2019;(2):11–18. DOI: 10.11603/2311-9624.2019.2.10158
Bila N, Shnaider SA, Safchuk K, Safarova L. Determination of the effect of hygienic rinses of various compositions on the state of the oral cavity microflora. Stomatol Bull. 2022;120(3):6–8. DOI: 10.35220/2078-8916-2022-45-3.2
Tartaglia GM, Tadakamadla SK, Connelly ST, Sforza C, Martín C. Adverse events associated with home use of mouthrinses: A systematic review. Ther Adv Drug Saf. 2019;10. DOI: 10.1177/2042098619854881
Selvaraj M, Mohaideen K, Sennimalai K, Gothankar GS, Arora G. Effect of oral environment on contemporary orthodontic materials and its clinical implications. J Orthod Sci. 2023;12(1):1. DOI: 10.4103/jos.jos_73_22
Ayesha S, Bhargava A, Philip AK, Sam G, Kumari D, George PP. Comparison of the antimicrobial activity of aloe vera mouthwash with chlorhexidine mouthwash in fixed orthodontic patients. J Contemp Dent Pract. 2022;23(7):743–48.
Zawadzki PJ, Perkowski K, Starościak B, Baltaza W, Padzik M, Pionkowski K, Chomicz L. Identification of infectious microbiota from oral cavity environment of various population group patients as a preventive approach to human health risk factors. Ann Agric Environ Med. 2016;23(4):566–69. DOI:10.5604/12321966.1226847
Zhou X, Li Y. Techniques for oral microbiology. In: Atlas of oral microbiology. Oxford: Academic Press; 2015. Chapter 2; p. 15–40. DOI: 10.1016/B978-0-12-802234-4.00002-1
Harley JP, Prescott LM. Laboratory exercises in microbiology. 5th ed. New York: The McGraw-Hill Companies; 2002. 480 p.
Kayalvizhi G, Radha S, Prathima GS, Mohandoss S, Ramesh V, Arumugam SB. Comparative evaluation of plaque removal effectiveness of manual and chewable toothbrushes in children: A randomized clinical trial. Int J Clin Pediatr Dent. 2019;12(2):107–10. DOI: 10.5005/jp-journals-10005-1604
Alroudhan EI, Gamal M, Ganji KK, Khan AM, Alsharari KN, Alruwaili MK, Al Waqdani NH. The effectiveness of mouthwashes with various ingredients in plaque control: A systematic review and meta-analysis. Alternative Ther Health Med. 2021;27(5):52–57.
Nawrot O. The biogenetical revolution of the Council of Europe – twenty years of the Convention on Human Rights and Biomedicine (Oviedo Convention). Life Sci Soc Policy. 2018;14:11. DOI: 10.1186/s40504-018-0073-2
Bescos R, Ashworth A, Cutler C, Brookes ZL, Belfield L, Rodiles A, et al. Effects of Chlorhexidine mouthwash on the oral microbiome. Sci Rep. 2020;10(1):5254. DOI: 10.1038/s41598-020-61912-4
Brookes ZLS, Belfield LA, Ashworth A, Casas-Agustench P, Raja M, Pollard AJ, Bescos R. Effects of chlorhexidine mouthwash on the oral microbiome. J Dent. 2021;113:103768. DOI: 10.1016/j.jdent.2021.103768
Chatzigiannidou I, Teughels W, Van de Wiele T, Boon N. Oral biofilms exposure to chlorhexidine results in altered microbial composition and metabolic profile. npj Biofilms Microbiomes. 2020;6:13. DOI: 10.1038/s41522-020-0124-3
Al-Kamel A, Baraniya D, Al-Hajj WA, Halboub E, Abdulrab S, et al. Subgingival microbiome of experimental gingivitis: Shifts associated with the use of chlorhexidine and N-acetyl cysteine mouthwashes. J Oral Microbiol. 2019;11(1):1608141. DOI: 10.1080/20002297.2019.1608141
Rajendiran M, Trivedi HM, Chen D, Gajendrareddy P, Chen L. Recent development of active ingredients in mouthwashes and toothpastes for periodontal diseases. Molecules. 2021;26(7):2001. DOI: 10.3390/molecules26072001
Albert-Kiszely A, Pjetursson BE, Salvi GE, Witt J, Hamilton A, Persson GR, Lang NP. Comparison of the effects of cetylpyridinium chloride with an essential oil mouth rinse on dental plaque and gingivitis – a six-month randomized controlled clinical trial. J Clin Periodontol. 2007;34(8):658–67. DOI:10.1111/j.1600-051X.2007.01103.x
Becker K, Brunello G, Scotti L, Drescher D, John G. Efficacy of 0.05% chlorhexidine and 0.05% cetylpyridinium chloride mouthwash to eliminate living bacteria on in situ collected biofilms: An in vitro study. Antibiotics. 2021;10(6):730. DOI: 10.3390/antibiotics10060730
Brookes Z, Teoh L, Cieplik F, Kumar P. Mouthwash effects on the oral microbiome: Are they good, bad, or balanced? Int Dent J. 2023;73(Suppl 2):74–81. DOI: 10.1016/j.identj.2023.08.010
Adam FA, Mohd N, Rani H, Mohd Yusof MYP, Baharin B. A systematic review and meta-analysis on the comparative effectiveness of Salvadora persica extract mouthwash with chlorhexidine gluconate in periodontal health. J Ethnopharmacol. 2023;302(Pt A):115863. DOI: 10.1016/j.jep.2022.115863
Newman BA, Rosebrough CN, Tamashiro RA, Dias Ribeiro AP, Whitlock JA, Sidhu G, et al. A randomized controlled trial to evaluate the effectiveness of a novel mouth rinse in patients with gingivitis. BMC Oral Health. 2022;22:461. DOI: 10.1186/s12903-022-02518-2
Chen I, Chung J, Vella R, Weinstock GM, Zhou Y, Jheon AH. Alterations in subgingival microbiota during full-fixed appliance orthodontic treatment – A prospective study. Orthod Craniofac Res. 2022;25(2):260–68. DOI: 10.1111/ocr.12534
Hussain U, Alam S, Rehman K, Antonoglou GN, Papageorgiou SN. Effects of chlorhexidine use on periodontal health during fixed appliance orthodontic treatment: A systematic review and meta-analysis. Eur J Orthod. 2023;45(1):103–14. DOI: 10.1093/ejo/cjac044
Langa GPJ, Muniz FWMG, Costa RDSA, Silveira TM, Rösing CK. The effect of cetylpyridinium chloride mouthrinse as adjunct to toothbrushing compared to placebo on interproximal plaque and gingival inflammation – a systematic review with meta-analyses. Clin Oral Investig. 2021;25:745–57. DOI:10.1007/s00784-020-03661-2
Oo MMT, Oo PH, Saddki N. Efficacy of 0.05% cetylpyridinium chloride mouthwash as an adjunct to toothbrushing compared with 0.12% chlorhexidine gluconate mouthwash in reducing dental plaque and gingival inflammation: A randomized control trial. Int J Dent Hyg. 2023;21(1):195–2. DOI: 10.1111/idh.12614
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