Morphological features of heart remodeling in the postcompression period of crush syndrome

Abstract

Summary. In the postcompression period of crush syndrome regenerative processes develop restorative processes in target organs, in particular in the heart. However, the dynamics of myocardial regenerative changes in the postcompression period in crush syndrome is insufficiently covered in professional literature and requires comprehension study and clarified.

The aim of the study – to find out features of regenerative changes in heart of laboratory rats in the dynamics in the postcompression period of crush syndrome.

Materials and Methods. Light-optical and polarization were performed examination of the myocardium of 48 laboratory rats after 1, 3, 7 and 14 days (12 animals in each period) after cessation of 6 hour compression of the thigh right pelvic limb. Crush syndrome was modeled under anesthesia by intraperitoneal input of ketamine hydrochloride (100 mg/kg body weight) in a specially designed device, the compression force was 7 kg/cm², the compressive surface area – 5 cm². The control group consisted of 12 animals.

Results. The stages of myocardial remodeling in the postcompression period are noted, reflecting its structural recovery. In the early postcompression period light-optical disorders predominated microcirculation and alternate changes in acquired cardiomyocytes maximum development after 3 days. After 7 days in myocardial stroma registered cellular infiltrates in which predominate lymphocytes and fybroblasts. Cardiomyocytes lose a compact, gait cells became fortuous, microfoci of contractures were detected and fragmentation, there was a weak proliferation of collagen fibers. After 14 days in the late postcompression period the manifestation do not fade microcirculation disorders. Processes were registered in some areas collagen formation.

Conclusions. In the first three days after the cessation of compression of compression of the lower extremity dystrophic–necrotic changes of the myocardium persist in combination with microcirculatory disorders. After 7 days the development of interstitial lympho–histiocytic infiltration is observed against the background of a gradual decrease alternative and hemodynamic processes. After 14 days registration a restoration of myocardial histostructure with residual processes of microfocal contracture of cardiomyocyte degeneration and increased collagen formation are registered.