CHANGES IN BIOCHEMICAL PARAMETERS OF NITROGEN RENAL FUNCTION OF RATS WITH LONG-TERM ADMINISTRATION OF THERAPEUTIC DOSES OF NALBUPHINE
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2021.1.12088Keywords:
rat, nalbuphine, kidney, urea, creatinine, daily diuresis, glomerular filtration rateAbstract
Summary. Given the problem of drug addiction, which is one of the most pressing problems of modern society, it is worth studying the morpho-functional state of the kidney with long-term use of opioids.
The aim of the study – to learn the changes in the biochemical parameters of nitrogen excretory function of the rat kidney with long-term administration of therapeutic doses of nalbuphine.
Materials and Methods. The studies were performed on 70 white sexually mature male rats weighing 180–260 g. Nalbuphine was administered to experimental animals in therapeutic doses for 12 weeks. Daily diuresis, urinary creatinine, serum creatinine, serum urea and glomerular filtration rate were determined every two weeks. The obtained data were tested for the normality of the distribution using the indicators of the asymmetry and excess coefficients, as well as using the Shapiro-Wilk test.
Results. Administration of therapeutic doses of nalbuphine for twelve weeks results in changes in the biochemical parameters of rat serum urea and creatinine, urine creatinine, daily diuresis and glomerular filtration rate. Significant, direct, pronounced correlations and maximal growth were found between serum urea and creatinine values at the sixth and eighth weeks of the experiment. There was a sharp decrease in glomerular filtration rate after four weeks of the experiment, the recovery of this indicator in the sixth week and the subsequent gradual decrease, which at the end of the twelfth week was significantly lower than in the control group. Urinary creatinine levels decreased sharply in the fourth week of the experiment, increased in the sixth and eighth weeks of the experiment and in the following periods decreased to the control group.
Conclusions. The results obtained indicate a change in the nitrogen excretory function of the kidney at different times of administration of therapeutic doses of nalbuphine.
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