MELATONIN EFFECT ON THE LIVER ANTIOXIDANT SYSTEM OF RATS WITH EXPERIMENTAL NEPHROPATHY
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2020.4.11802Keywords:
experimental nephropathy, antioxidant system, liver, melatonin, glutathioneAbstract
Summary. Kidney lesion results in complications of many somatic diseases and increases the risk of death of patients determining the necessity to find means of effective and safe pharmacological correction of nephropathy of various etiologies. The liver and kidneys play an important role in neutralization of xenobiotics, therefore they are more inclined to oxidative damage. Melatonin is a hormone secreted by the pineal gland possessing anti-inflammatory and anti-oxidant properties. Examination of its effects produced on the antioxidant liver system in case of kidney diseases is reasonable.
The aim of the study – to assess melatonin effect on the liver antioxidant system of rats with experimental nephropathy.
Materials and Methods. The experiment was conducted on 127 nonlinear male rats with the body weight of 0.16–0.18 kg. Experimental nephropathy was simulated by means of a single injection of folic acid into the peritoneum in the dose of 250 mg/kg of the body weight. Melatonin was introduced into the stomach during 3 and 7 days after nephropathy simulation in the dose of 10 mg/kg. The state of the liver antioxidant system was assessed by the parameters of SOD, catalase, GP, GST activity and the content of reduced glutathione in the post-mitochondrial liver fraction.
Results. Considerable disorders of the antioxidant system examined parameters were found in the liver of animals with experimental nephropathy. Thus, GP, GST activity, the content of reduced glutathione decreased, and the activity of SOD and catalase in the liver of rats with nephropathy increased on the 3d day of experiment versus similar parameters of the control group. Melatonin introduction under conditions of nephropathy promoted normalizing SOD, catalase, GST activity and the content of reduced glutathione on the 7th day of the experiment, though GP activity remained reduced.
Conclusions. Imbalance of the antioxidant system parameters is found in animals with experimental nephropathy, which might be caused by intensified free radical damage of the biomolecules. Melatonin introduction during 7 days promotes normalizing of the antioxidant system parameters in the liver of rats with nephropathy, which might be stipulated by its ability to directly neutralize toxic compounds and activate antioxidant enzymes.
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