INFLUENCE OF THE CARRAGEENAN AND SODIUM GLUTAMATE COMBINED ACTION ON HEMOPOESIS INDICIES
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2020.4.11800Keywords:
κ-карагінан, натрій глутамат, комбінація, еритроцити, гемоглобін, ретикулоцитиAbstract
Summary. Despite the large number of scientific indicators to support the safety of glutamate sodium and carrageenan, some researchers provide preliminary information on the safety of their use, which creates the preconditions for an in-depth study of their combined effects in the experimental environment.
The aim of the study – to analyze the indicies of hematopoiesis in animals in case of experimental usage of carrageenan solution, monosodium glutamate and their combined effect.
Materials and Methods. The study was performed on 48 white nonlinear male rats, which were divided into 4 groups: 1 – control (intact animals), 2 – animals that were intragastrically administered carrageenan at a dose of 40 mg/kg during 1 month, 3 – animals that were intragastrically administered monosodium glutamate at a dose of 50 mg/kg during 1 month, 4 – animals that were intragastrically administered combination of carrageenan and monosodium glutamate in the same doses. The erythrocytes level is carried out with the help of a microscope in counting chambers with a Goryaev grid, determination of blood hemoglobin by hemiglobiancyanide method. To count reticulocytes in 0.3 ml of blood, 100 μl of 1 % diamond cresyl blue dye was added, the count was performed using a microscope. The concentration of erythropoietin (EPO) was determined by enzyme-linked immunosorbent assay.
Results. In the study of red blood cell counts and EPO concentrations in animals of groups 1 and 2, no pathological changes was occurred. It was found that the level of hemoglobin in the blood of all studied groups was significantly different when analyzing the rank variations of Kruskal-Wallis. Prior to that, in animals, using the combined administration of carrageenan and monosodium glutamate, the number of erythrocytes and hemoglobin was significantly lower than both experimental groups 1 and 2, and the control one. The concentration of EPO in experimental group 3 exceeded the value of this indicator both in the control (32.05 %) and in experimental group 1 (34.64 %) and 2 (32.61 %).
Conclusions. The combined usage of κ-carrageenan and monosodium glutamate solutions has a negative effect on hematopoiesis, which is manifested by a statistically significant decrease in erythrocytes and hemoglobin levels and an increase in the concentration of erythropoietin.
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