Changes of indices of variation cardiointervalometry in male rats with castration and stress in the development of adrenaline heart damage
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2022.4.13318Keywords:
autonomic nervous system, variational cardiointervalometry, adrenaline, heart, stress, castrationAbstract
Summary. Studying the role of the autonomic nervous system in the effect of stress on the body is relevant. The catecholamines released in this case cause disturbances in the work of the heart and contribute to the development of cardiovascular pathology. Stress also causes suppression of testosterone synthesis and spermatogenesis.
The aim of the study – to analyze changes in variational cardiointervalometry indicators in castrated and stressed rats during the development of adrenaline heart damage (AHD).
Materials and Methods. The study was performed on 240 white male Wistar rats. The animals were divided into four series: 1 – control, 2 – stress, 3 – castration, 4 – castration and stress. For AHD, rats were injected once intramuscularly with a 0.18 % solution of adrenaline hydrotartrate at the rate of 0.5 mg/kg of weight. Stress was induced from 1.5 to 3 months of age by constant housing in cages with a limitation of living space twice. The method of variational cardiointervalometry was used to study heart rate variability. Castration and ECG recording were performed under thiopental-sodium anesthesia. ECG was recorded in control, 1, 3, 7, 14 and 28 days after AHD.
Results. During the analysis of variational cardiointervalometry indices, changes were noted in the control groups, indicating adaptation to the simulated conditions. In 1 series of animals, 3 days after the development of AHD, there were larger changes in fashion (Mo). After 1 day, compared to the control, the amplitude of the mode (AMo) decreased by 57.5 % (p<0.05), the stress index (SI) by 2.3 times (p<0.05), the variation score increased (ΔX ) by 46 % (p<0.05). In the 2nd series, the most significant changes were observed after 28 days (ΔX increased by 40 %, heart rate decreased by 10.4 % and SI by 2.3 times). In the 3rd series, after 1 day, reliable changes in all indicators were noted. After 3 days, the indicators improved significantly, but after 7 days they worsened again and remained so until the end of the experiment. In series 4, the worst periods were the periods after 7 days (Mo increased by 27.4 %, AMo decreased by 80.3 %, ΔX increased by 69.8 %, HR decreased by 27.6 % and SI by 4.1 times) and after 28 days (Mo increased by 12 %, AMo decreased by 48.6 %, ΔX increased by 23.8 %, heart rate decreased by 12.3 % and SI by 2.2 times).
Conclusions. The difference in ensuring the autonomous regulation of the heart rhythm in animals that have stress and castration, which can cause the greatest damage to the heart in case of excessive release of adrenaline, has been revealed. For animals that are in normal conditions – until the end of 3 days; for animals that have hypodynamic lifestyle – 28 days; for animals that have castration – 1 day, and the period from 7 to 28 days. In animals with combined pathology, the 7th and 28th days are the most vulnerable.
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