Changes in lipid peroxidation and antioxidant system parameters in the liver of rats using mexidol in experimental obesity and bronchial asthma
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2021.4.12566Keywords:
bronchial asthma, obesity, LPO, AOS, MexidolAbstract
Summary. The problem of bronchial asthma (BA) prevention and treatment in combination with obesity can be solved only on the basis of a deep understanding of the biochemical and physiological mechanisms of two pathologies formation. Among the biochemical mechanisms underlying their development, the processes of free radical and peroxidation occupy a special place. The lungs and liver are the main target organs in the combination of ВA and obesity pathologies. However, despite the prevalence of these diseases, today's science has rather insignificant data on the state of LPO-AOS of the liver specifically in BA and obesity, and especially the effect of antioxidants on it, which requires study.
The aim of the study – to determine the parameters of LPO-AOS in the liver of rats in an experimental model of obesity and ВA and the effect of Mexidol on them.
Materials and Methods. The study included 20 white non-linear sexually mature rats. Experimental animals were divided into 4 groups: intact – control group (5 rats), group 1 – observation group on day 15 without Mexidol correction (5 rats), group 2 – observation group on day 26 without Mexidol correction (5 rats) and group 3 – observation group on day 26 after Mexidol correction experiment (5 rats). The content of malondialdehyde, conjugated diene, superoxide dismutase, catalase activity, and ceruloplasmin content were studied.
Results. In experimental groups 1 and 2 the LPO parameters increased (p<0.05). Decrease in the activity of the enzymatic link of AOS, but still including the protective reaction of hepatocytes against LPO products explains the increase in the content of superoxide dismutase and catalase in the experimental group 1, but already in the experimental group 2 their concentration decreased by 17 % and 10 % (p<0.05), respectively, in comparison with intact animals. This decrease in superoxide dismutase and catalase on day 26 of the experiment without correction with Mexidol is explained by depletion of liver AOS. As well as enzymes, the content of ceruloplasmin changed similarly. The third group of animals studied confirmed the antioxidant activity in the liver tissue of Mexidol preparation, because both diene conjugates and malonicdyaldehyde values decreased and AOS values increased.
Conclusions. The study of the liver tissue confirmed the efficacy of the use of Mexidol for the regulation of LPO-AOS parameters in the combined pathology of ВA and obesity.
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