ACTIVITY OF THE MICROSOMAL OXIDATION SYSTEM IN RAT LIVER UNDER THE INFLUENCE OF SODIUM FLUORIDE

Abstract

Summary. The pathogenesis of fluoride intoxication at the molecular, cellular and functional levels has not been sufficiently studied. There are very few modern data on these issues, so they are contradictory, since the effects of this trace element are multifaceted and cannot be characterized unambiguously.

The aim of the study – to learn the state of the monooxygenase system of rat hepatocytes under conditions of the formation of fluoride intoxication.

Materials and Methods. In the experiment, we used 30 sexually mature rats (N=30) of the Wistar population weighing 200–210 g for 1.5 months. Sodium fluoride solution was administered orally at doses of 1/10 DL50, which was 20 mg/kg of animal body weight.

Results. The results of experiments on the study of oxygen consumption by rat liver microsomes under fluoride intoxication indicated that the rate of endogenous respiration of microsomes, the rate of NADPH oxidation, the rate of NADH oxidation in the presence of EDTA, and the rate of lipid peroxidation increase under the influence of fluorides. Sodium fluoride stimulated an increase in all parameters of microsomal oxidation, except for cytochrome b5. It should be assumed that in this case there is an increase in the generation of reactive oxygen species, free radicals, which stimulate the development of free radical processes in the body and are, most likely, the leading link in oxidative stress.

Conclusions. These changes indicate a violation of the bioenergetics of hepatocytes associated with the mitochondrial apparatus and the development of hypoxic processes, which lead to a decrease in the activity of redox reactions occurring at the level of intracellular membranes and organelles.