THE INFLUENCE OF THIOTRIAZOLINE ON THE PRIMARY AND SECONDARY IMMUNE RESPONSE IN THE FORMATION OF CONTACT DERMATITIS AND PNEUMONIA

Abstract

Summary. Contact dermatitis (CD) and pneumonia remain the most common diseases among the pathology of the bronchopulmonary system and skin.

The aim of the study – to determine the level of activity of immunoglobulins A, M, G in the blood and to establish the corrective effect of thiotriazoline on them in the conditions of CD and experimental pneumonia (EP).

Materials and Methods. Studies were performed on 55 guinea pigs, males, weighing 180–210 g. Contact dermatitis was reproduced by the method of V. A. Volkova, EP was reproduced by the method of V. N. Shlyapnikov,  T. L. Solodova. The content of immunoglobulins A, M, G in the blood was determined by the method of E. F. Chernushenko, L. S. Kogosova. Thiotriazoline was administered daily at a dose of 100 mg per 1 kg of body weight for 10 days (from the 8^th to the 18^th day) EP and CD. Animals were decapitated under ether anesthesia on the 4 ^th, 8 ^th, 10 ^th and 18 ^th days before and after administration of this drug.

Results. It was found that CD and EP (4 ^th, 8 ^th, 10 ^th and 18 ^th days) is accompanied by an increase in Ig A content in the blood by 46.4 % (p <0.05), 84.6 % (p <0.05), 115.4 % (p <0.001) and 123.1 % (p <0.001). Ig M by 50.0 % (p <0.05), 58.3 % (p <0.05), 91.7 % (p <0.05) and 103.3 % (p <0.001), respectively; Ig G by 49.0 % (p <0.05), 73.6 % (p <0.05), 86.8 % (p <0.05) and 90.6 % (p <0.05) against control before treatment. The use of thiotriazoline led to a decrease in Ig A, Ig M and Ig G in the blood by 27.5 % (p <0.05), 36.0 %  (p <0.05) and 29.7 % (p <0, 05) in relation to a group of animals with EP and CD before treatment, which indicates its immunocorrective effect.

Conclusions. Contact dermatitis and experimental pneumonia are accompanied by a gradual increase in the level of Ig A, M, G in the blood throughout the period of their development with dominance in the latest period of observation (10^th and 18^th days), which gives grounds to state their active participation in the mechanisms of formation primary and secondary immune response. The use of thiotriazoline caused an immunocorrective effect on the level of immunoglobulins in the blood in CD and EP.