SIGNIFICANCE OF MIDDLE MASS MOLECULES IN PROGNOSIS ASSESSMENT OF ETHANOL LIVER DAMAGE IN RATS WITH DIFFERENT EMOTIONALITY

Abstract

Summary. Alcoholic liver damage is a major economic and demographic problem worldwide. One of the syndromes that always accompany liver damage is endogenous intoxication, the presence of which can be determined by the content of middle mass molecules (MMM). The level of MMM depends on the body's metabolism and is a prognostic criterion for metabolic disorders.

The aim of the study – to determine the degree of endogenous intoxication by the content of middle mass molecules in the serum of male rats with varying degrees of emotionality in ethanol liver damage.

Materials and Methods. The experiments were performed on 192 white outbred high- and low-emotional male rats (HE and LE). The emotionality of rats was determined by the method of "open field". All rats were divided into the following groups: control, glucose 7 days, ethanol hepatitis (EG); glucose 60 days, ethanol hepatosis (EGs), ethanol fibrosis (EF), ethanol cirrhosis (EC). The level of intoxication in the serum was determined by the content of molecules of middle mass at wavelengths λ = 238, 254, 260 and 280 nm.

Results. The greatest accumulation of MMM₂₃₈ was with EGs in both HE and LE, and then with EG, more dominant in LE, with EF – in HE and EC, regardless of emotionality. The greatest accumulation of MMM₂₅₄ was at EGs, more at HE, then at EG regardless of emotionality, at EC and EF – higher in HE and at EF and EC – higher in LE. The greatest accumulation of MMM₂₆₀ was at EGs, more at HE, then at EC in both HE and LE, at EF, more at HE and at EG regardless of emotionality. The largest accumulation of MMM₂₈₀ in HE animals was in the following order EG> EC> EF = EGs, in LE – EG> EC> EGs> EF.

Conclusions. A study of endogenous intoxication on the content of MMM showed that ethanol has a toxic effect on the body. The destruction of hepatocytes decreases as the pathological process deepens from hepatosis to liver cirrhosis. Dependence on the emotional state of the animal in the development of ethanol damage to the body is manifested only in hepatitis and fibrosis. The largest accumulation of toxic substances is as follows: in highly emotional animals – hepatosis> hepatitis> fibrosis = cirrhosis, in low-emotional animals – hepatitis> hepatosis> fibrosis> cirrhosis.