INFLUENCE OF ACUTE BLOOD LOSS COMPLICATED BY LIMB ISCHEMIA-REPERFUSION ON THE ACTIVITY OF LIPID PEROXIDATION PROCESSES IN LUNGS AND THEIR CORRECTION WITH CARBACETAM
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2020.3.11525Keywords:
acute blood loss, ischemia-reperfusion of the limb, reagents for thiobarbituric acid, lungs, carbocetamAbstract
Summary. Recently, there has been a significant increase in the frequency of terrorist attacks and local armed conflicts. Their characteristic feature was an increase in the number of wounded with massive external bleeding, which require immediate use of a tourniquet with the maximum safe period of complete ischemia of the limb up to 2 hours. It is proved that ischemia of the limb and acute blood loss contribute to the initiation of lipid peroxidation due to increased production of reactive oxygen species.
The aim of the study – to determine the effect of acute blood loss complicated by limb ischemia-reperfusion on the intensity of lipid peroxidation of the lungs and the effectiveness of correction of detected disorders by carbacetam.
Materials and Methods. The study was performed on 216 white nonlinear male rats weighing 200–220 g. All experiments were performed under thiopental-sodium anesthesia. In animals, limb ischemia-reperfusion and acute blood loss were simulated and these lesions were combined. In a separate group, the detected disorders were corrected with carbacetam. After 1 and 2 hours, as well as after 1, 7 and 14 days in experimental animals, the content of reagents to thiobarbituric acid was determined, which belongs to the screening indicators of the activity of lipid peroxidation processes.
Results. Under conditions of ischemia-reperfusion of the limb, acute blood loss and their combination in the lungs increases the content of reagents to thiobarbituric acid. If after modeling only ischemia-reperfusion of the limb the figure is normalized up to 14 days, then after modeling acute blood loss and its complication by ischemia-reperfusion of the limb, the figure increases with the first maximum after 1 day and the second after 14 days and significantly exceeds the control level. Complications of acute blood loss by ischemia-reperfusion of the limb at all times is accompanied by a higher content of reagents to thiobarbituric acid compared to other experimental groups, which suggests a mutual burden with the depletion of antioxidant protection factors. Under the influence of carbacetam in all experimental groups the indicator is statistically significantly reduced, which indicates a pronounced antioxidant effect of the drug. Although the indicators did not reach the level of the control group, carbacetam can be considered a promising means of correction of prooxidant disorders in the lungs in conditions of acute blood loss complicated by ischemia-reperfusion of the limb.
Conclusions. Under conditions of ischemia-reperfusion of the limb, acute blood loss and their combination in the lungs, the processes of lipid peroxidation are intensified, which is manifested by an increase in the content of reagents to thiobarbituric acid. Due to the simulation of acute blood loss complicated by ischemia-reperfusion of the limb, the violations are greater in all terms of observation. The use of carbacetam after 7–14 days reduces the content of reagents to thiobarbituric acid in all experimental groups, which indicates the prospects of the tool for the correction of detected disorders.
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