CHANGES OF MACRO- AND MICROELEMENT COMPOSITION OF RAT LIVER IN THE DYNAMICS OF CRUSH-SYNDROME DEVELOPMENT
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2020.3.11524Keywords:
crush-syndrome, calcium, magnesium, zinc, cuprum, liver, experimentAbstract
Summary. Given the high lethality in severe forms of crush-syndrome, the study of mechanisms in the dynamics of a given pathological syndrome is of great importance.
The aim of the study – to investigate the dynamics of macro- and micronutrient composition of rat liver in a model of endotoxicosis, which is formed under conditions of crush-syndrome.
Materials and Methods. Experiments were conducted on 40 adult white male rats. The experimental model was a pathological process that developed in animals as a result of the compression of the soft tissues of the left pelvic limb for 4 hours in a special device. The area of the compressive surface was 4 cm2, and the force of compression was 4.25 kg/cm2. At the same time, the integrity of large vessels and bone structures of the lower limb was maintained. Thus, a moderate degree of crush-syndrome was simulated. In the liver of experimental animals, the level of macro- and microelements was determined by atomic absorption spectrophotometry using a C-115 PC spectrophotometer.
Results. Thus, calcium content in the liver increased in 3rd day of experiment by 18.2 % and remained high until 14 days. At the same time, magnesium levels decreased by 19.2 % at first day, fluctuating within these values to 7th day, and then decreased again by 106.7 % relative to the control group. Zinc content in the liver gradually increased from first day (by 12.8 %) to 14th day (by 34.0 %) of the observation relative to control. The same trend was observed for the content of cuprum, but its level probably increased at 3rd day of the experiment.
Conclusions. In conditions of hypoxia and oxidative stress, which develop in the tissues of rats with experimental crush-syndrome, there is an imbalance of macro- and microelements, which is characterized by an increase in calcium, zinc and cuprum content and a decrease in the magnesium content in the liver of animals.
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