PECULIARITIES OF SERUM CYTOKINE PROFILE IN RATS IN MUSCLE INJURY DURING PLATELET-RICH PLASMA CORRECTION
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2020.3.11518Keywords:
muscle injury, cytokines, platelet-rich plasmaAbstract
Summary. Platelet-rich plasma have become increasingly relevant in the last decade and are increasingly the focus of experimental and clinical research in the context of wound healing and tissue regeneration. However, despite various programs, the effectiveness of regenerative methods of treatment of platelet-rich blood plasma remains relevant.
The aim of the study – to evaluate the efficacy of platelet-rich plasma on cytokine profile in the setting of experimental muscle injury.
Materials and Methods. The experimental study was performed on 92 nonlinear white rats. The injury was modeled according to the developed method in the conditions of thiopental-sodium anesthesia. Obtaining platelet-rich plasma occurred in two stages with all the rules of sterility. Determination of serum cytokines (TNFα, IL1β, IL6, IL10) by solid-phase enzyme-linked immunosorbent assay.
Results. Serum proinflammatory cytokines in rats muscle injury were significantly higher than in animals without simulated pathology. The use of platelet-rich blood plasma (PRP) was accompanied by a decrease in the concentration of proinflammatory serum cytokines. Comparing the data obtained before and after correction, we can conclude about the anti-inflammatory effect of PRP, which was characterized by a decrease in the concentration of proinflammatory cytokines, which are strongly produced in the inflammatory process in response to muscle injury at all times, and hyperproduction of anti-inflammatory IL10, which can inhibit TNFα production and mitigate its negative effects.
Conclusions. Thus, in animals with mechanical injury to the thigh muscles, there is an increase in the concentration of IL1β, TNFα and IL6 in the serum against the background of depletion of anti-inflammatory reserves. The use of PRP reduces the proinflammatory effect of cytokines by reducing their concentration from the first day of the experiment.
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