QUANTITATIVE MORPHOLOGICAL ANALYSIS OF THE STRUCTURAL RECONSTRUCTION OF THE HEMOMICROCIRCULATORY BED OF THE COMMON BILE DUCT IN RESECTIONS OF DIFFERENT VOLUMES OF THE LIVER
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2020.3.11516Keywords:
postresection portal hypertension, common bile duct, hemomicrocirculatory bedAbstract
Summary. Removal of large volumes of liver leads to portal hypertension, bleeding from varicose veins of esophagus, stomach, rectum, ascites, splenomegaly and structural reconstruction of organs of the hepatic portal vein and the common bile duct, in which the morphology of hemomicrocirculatory tract in resection of various volumes of liver is insufficiently studied.
The aim of the study – morphometrically to study the features of the structural reconstruction of vessels of hemomicrocirculatory bed of the common bile duct in resection of different volumes of the liver.
Materials and Methods. The research was conducted on 60 male lab rats, which were divided into 4 groups. Group 1 – 15 intact animals, 2 – 15 rats after removal of 31.5 % of the liver parenchyma, 3 – 15 animals after resection of 42.0 % of the volume of liver, 4 – 15 rats after removal of 58.1% of the liver parenchyma. Euthanasia of animals was performed by bloodletting under thiopental anesthesia 1 month from the beginning of the experiment. Micropreparations were made from the common bile duct, and in part of the observations the hemomicrocirculatory bed was poured with mascara-gelatin mixture. The diameters of arterioles, precapillary arterioles, hemocapillaries, capillary venules, venules, microvascular density, relative volume of damaged endothelial cells were determined morphometrically. Quantitative indicators were processed statistically
Results. Removal of 58.1 % of liver parenchyma leads to postresection portal hypertension and structural reconstruction of vessels of the hemomicrocirculatory bed of the common bile duct, which was characterized by pronounced narrowing of the lumens of arterioles, precapillary arterioles, hemocapillaries, significant expansion of capillary venules and venules, decreased microvascular density, endothelial cell damage.
Conclusions. Resection of 58.1 % of the liver parenchyma leads to postresection portal hypertension, severe narrowing of arterioles, precapillary arterioles, hemocapillaries and expansion of capillary venules and venules of the common bile duct. Dilation of venous vessels of the hemomicrocirculatory bed leads to venous plethora, hypoxia, endothelial cell damage, endothelial dysfunction, perivasal and stromal edema, dystrophy, necrobiosis of cells, tissues, infiltrative and sclerotic processes.
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