DYNAMICS OF CHANGES OF THE MINERAL COMPOSITION OF MICRO- AND MACROELEMENTS OF BONE TISSUE OF THE TEMPOROMANDIBULAR JOINTS IN DIABETES
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2020.3.11358Keywords:
microelements, macroelements, diabetes mellitus, streptozotocin, temporomandibular joint, spectrophotometryAbstract
Summary. Diabetes mellitus (DM) is widespread in many countries of the world. It influences on mineral metabolism not only throughout the body but also in bone tissue in particular, which leads to osteoporosis, and therefore to frequent fractures, early disability and mortality. However, there is no data in the scientific literature on changes in the minerals of the temporomandibular joints (TMJ) in diabetes.
The aim of the study – to establish the dynamics of changes in the mineral composition of micro- and macroelements of TMJ bone tissue in diabetes mellitus.
Materials and Methods. The experimental study was performed on 90 white adult male rats weighing 90–320 g, which were kept in the diet of the vivarium. The animals were divided into 6 groups of 15 rats each. Males of the groups 1, 2, 3 were simulated diabetes mellitus by a single injection of streptozotocin "Sigma". Rats of the groups 4, 5, 6 were used as controls at the appropriate time of the study. Animals were slaughtered 1, 2 and 3 months after the start of the experiment, followed by sampling TMJ complexes and adjacent soft tissues from both sides. For spectrophotometry of bone ash TMJ was prepared according to the method of O. H. Babenko. For analysis, the resulting ash was dissolved in 10 % hydrochloric acid and nitric acids and adjusted with double-distilled water up to 25 ml. Flammable nuclear computer system absorption spectrophotometer C-115 calculates concentrations of macro- and microelements in experimental solutions. The phosphorus content was determined on FEC-2 by the method Briggs.
Results. Data from spectrophotometric studies of rat TMJ indicate a decrease in the level of inorganic substances in bone tissue while maintaining the amount of organic matter and a significant increase in the degree of hydration. Along with this, there is an increase in the level of some trace elements (potassium, sodium, magnesium) and a decrease in others (calcium, phosphorus, copper, manganese).
Conclusions. Demineralization of the TMJ bone tissue of rats with diabetes, which is manifested in changes in the levels of macro- and microelements, leads to the development of osteoporosis, and therefore to destructive diseases of these joints.
References
Pestun IS. Features of reparative osteogenesis in tubular bone fractures in patients with diabetes. Master's thesis, Sumy State University; 2016. Ukrainian.
Juravliova LV, Oliinyk MO. Changes in bone metabolism in patients with osteoarthritis and its combination with type 2 diabetes mellitus. Achievements and prospects of experimental and clinical endocrinology (Fifteen Danilevsky’s readings): materials of the scientific-practical conference with international participation, Kharkiv, March 10-11, 2016; SI Institute of Endocrine Pathology. V.Ya. Danilevsky National Academy of Medical Sciences of Ukraine, Kharkiv National Medical University, Kharkiv Medical Academy of Postgraduate Education of the Ministry of Health of Ukraine. Kharkiv, 2016. Ukrainian.
Khandoga VS. Radiation diagnosis of changes in the skeleton of the lower extremities in diabetes mellitus Master's thesis. Sumy: Sumy State University; Med. Inst. 2016. Ukrainian.
Germanchuk SM. [Changes in the alveolar sprout in the chronic phase of simulated diabetes mellitus (for 3 months)]. Ukr zhurn med, boil i sport. 2018;(3, 2): 21-5. Ukrainian. DOI: https://doi.org/10.26693/jmbs03.01.026
Safarova SS. [Evaluation of bone remodeling in patients with type 2 diabetes mellitus]. Zaporozh med zhurn. 2019;(21,1): 60-3. DOI: 10.14739/2310–1210.2019.1.155812. Russian. DOI: https://doi.org/10.14739/2310-1210.2019.1.155812
Sykal AA. [Bone mineral density in women with type 2 diabetes]. Current areas of research: from theory to practice. 2015;(1): 60-3. Russian.
Khvisyuk AN, Sykal AA, Babalyan VA, Kalchenko AV. [Fracture risk assessment in patients with diabetes mellitus]. Ortop, travm i prop. 2015;(2): 35-41. Russian. DOI: https://doi.org/10.15674/0030-59872015235-41
Abramov AV, Ganchev KS. [Quantitative analysis of proliferative activity of rats’ periodontal tissues after tooth extraction on the background of experimental diabetes mellitus]. Klin i eksperym patol. 2019;(18,(1): 10-5. DOI: 10.24061/1727– 4338.XVІІI.1.67.2019.199. Ukrainian.
Hopta NS. Influence of cadmium salts and nitrites on metabolism in bone tissue. Candidate’s thesis. National Academy of Agrarian Sciences of Ukraine, Institute of Biology of Animals. 2015. Ukrainian.
Yakymets MM. Morphofunctional features of periodontal remodeling in hyperglycemia (clinical-experimental study). Candidate’s thesis. I.Ya. Horbachevsky Ternopil State Medical University. 2011. Ukrainian.
