SIGNIFICANCE OF ENDOGENOUS INTOXICATION MARKERS IN PROGNOSIS ASSESSMENT OF ISCHEMIC REPERFUSION LIMB SYNDROME
УДК 616-008.6:616.748-005.4]-092.9
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2020.3.11357Keywords:
ischemia, reperfusion, blood loss, endogenous intoxication, erythrocyte intoxication index, experimentAbstract
Summary. The use of hemostatic tourniquet is a proved means of primary care. However, systemic disorders, as well as ultrastructural, in the area of compression can significantly worsen the condition of the injured body. Establishing the severity of endogenous intoxication is an important aspect of understanding the course of ischemic-reperfusion syndrome (IRS) of the limb.
The aim of the study – to investigate the level of erythrocyte intoxication index (EII) in different periods of IRS development.
Materials and Methods. 260 white adult male rats were used in the experiment. They were divided into 6 groups: control, EG1 – simulation of isolated IRS of the limb), EG2 – simulation of isolated volumetric blood loss, EG3 – combination of IRS of the limb with blood loss, EG4 – simulation of isolated mechanical injury of the thigh, EG5 – combination of IRS of the limb and mechanical injury.
Results. It was found that each of the experimental interventions has led to an increase of investigated index. The peculiarity was that in the first hour after decompression EII on the background of isolated blood loss was the highest, but by the 7th day it has decreased. In this case, in the period from 1 to 3 days inclusive was at the level of EII levels on the background of IRS, combined with mechanical trauma. The most expressed development of intoxication syndrome was found in the group, where IRS was combined with blood loss. Thus, on the 3rd day – the index exceeded the initial level by 2.1 times % (p<0.05), on the 14th day remained elevated, compared with the control – by 77.7 % (p <0.05). Also, on the 3rd day the index in this group was the highest, comparatively to other explored periods.
Conclusions. The combination of ischemic-reperfusion syndrome with blood loss led to severe endogenous intoxication, and the resulting disorders were most expressed in this group, compared with the isolated effects of each experimental intervention.
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