KEY FUNCTIONAL CHANGES IN MESENTERIC LYMPHATIC NODE CELLS IN THE POSTERITY OF RATS WITH EXPERIMENTAL GESTATIONAL DIABETES

Authors

  • T. M. Prozorova Zaporizhzhia State Medical University
  • K. S. Krupey Zaporizhzhia State Medical University
  • O. M. Kamyshnyi Zaporizhzhia State Medical University

DOI:

https://doi.org/10.11603/bmbr.2706-6290.2020.1.11118

Keywords:

experimental gestational diabetes, mesenteric lymph nodes, innate immunity

Abstract

Gestational diabetes (GD) is an important metabolic disorder that can affect the morphogenesis of the immune system.

The aim of the study – to find out the peculiarities of mesenteric lymph nodes cells functional state in the posterity of rats with experimental gestational diabetes (EGD).

Materials and Methods. Studies were performed on 80 posterity of Wistar rats with EGD at 1 and 6 months of age. For the induction of EGD streptozotocin (SIGMA Chemical, USA) was administered intraperitoneally at a dose of 45 mg/kg. For identification of TLR2, TLR4, NOD2, RIGI, T-bet, Nlrp3, RORγt and Foxp3 in histological sections of lymph nodes immunofluorescence method was used. To study the mRNA expression of Aire, Deaf1, Foxp3, IL10, Ctla4, Cxcr4, Ccr7, Madcam1, S1pr1 genes, a real-time reverse transcription polymerase chain reaction method was used.

Results. The complex of key pathophysiological and functional changes in cells of mesenteric lymph nodes (MLN) in the posterity of rats with EGD was established: changes in the expression of lymphocyte recycling regulators and homemaking; impaired formation of peripheral immunological tolerance; activation of patterns of recognition receptors of the innate immune system on MLN lymphocytes; changes in the distribution of effector T cells in MLN.

Conclusions. Prenatal hyperglycemia leads to increased proinflammatory signaling and activation of innate immune system components more clearly at 1 month of life.

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Published

2020-05-23

How to Cite

Prozorova, T. M., Krupey, K. S., & Kamyshnyi, O. M. (2020). KEY FUNCTIONAL CHANGES IN MESENTERIC LYMPHATIC NODE CELLS IN THE POSTERITY OF RATS WITH EXPERIMENTAL GESTATIONAL DIABETES. Bulletin of Medical and Biological Research, (1), 36–41. https://doi.org/10.11603/bmbr.2706-6290.2020.1.11118

Issue

No. 1 (2020): Bulletin of Medical and Biological Research

Section

Original research
Received 2020-05-23
Accepted 2020-05-23
Published 2020-05-23