THE CHANGES OF CYTOKINE PROFILE IN RATS WITH MODELED SECONDHAND TOBACCO SMOKING COMBINED WITH PROLONGED ADMINISTRATION OF MONOSODIUM GLUTAMATE IN THE AGE ASPECT
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2020.1.10899Keywords:
secondhand tobacco smoke, monosodium glutamate, age, cytokinesAbstract
The World Health Organization considers tobacco smoke as global health and social problem. The active use of dietary supplements across the whole food industry is ambiguous due to human health and safety. So, the study of molecular mechanisms of toxicity of isolated and combined action of tobacco smoking and monosodium glutamate is one of the topical issues of modern science.
The aim of the study – to determine changes in the cytokine profile of blood serum in rats under secondhand tobacco smoking combined with a long-term monosodium glutamate injection in the age aspect.
Materials and Methods. Experiments were performed on 64 outbred white male rats divided into the following groups: I – control group; II – rats with modeled secondhand tobacco smoking; III – rats, which were injected with monosodium glutamate; IV – rats with modeled secondhand tobacco smoking combined with the monosodium glutamate injection. Blood serum cytokine content was performed by enzyme-linked immunosorbent assay (ELISA) Kit developed by RayBiotech (USA) due to the manufacturer's protocol on a Multiscan FC analyzer (Finland).
Results. We have established a pronounced increase of IL1β (in 2.2-times (p<0.001)) in mature rats in case of secondhand tobacco smoking combined with monosodium glutamate injection concerning the control group. This did not differ from the same indicator due to the isolated effect of tobacco smoke. TNFα increased more pronouncedly (in 2.9-times (p<0.001)) concerning the control group and 32.3 % (p<0.002) exceeded the data due to the isolated effect of tobacco smoke. A pronounced decrease of the anti-inflammatory cytokine IL10 to 61.0 % (p<0.001) concerning the control group was established. This was 29.7 % (p<0.001) below a similar indicator due to the isolated effect of tobacco smoke. In the age aspect, changes in blood serum cytokine profile in immature rats are more pronounced.
Conclusions. Secondhand tobacco smoke combined with monosodium glutamate injection is accompanied by more pronounced dyscytokinemia. It was confirmed by the probable increase of proinflammatory cytokines (TNFα) combined with the decrease of anti-inflammatory cytokines (IL10) compared to the data under the isolated action of tobacco smoke. In the age aspect under passive tobacco smoking combined with monosodium glutamate injection, intensive changes in the cytokines TNFα, IL1β and IL10 in the blood serum of immature rats exceed indexes of mature on 141.9; 70.4 and 16.1 %, respectively.
References
Jha P, MacLennan M, Chaloupka FJ, Yurekli A, Ramasundarahettige C, Palipudi K, Zatońksi W, et al. Global hazards of tobacco and the benefits of smoking cessation and tobacco taxes. Disease Control Priorities. The World Bank. 2015;3: 175-94.
World Health Organization. WHO report on the global tobacco epidemic 2019: Offer help to quit tobacco use.
Statistics Committee of Ukraine. Self-assessment of effective health and affordability of health care growth in 2008 [Electronic resource]. Available from: http: // www. ukrstat. gov. ua operativ2009. html. Ukrainian.
[Global survey of adults on tobacco use] (Global Adult Tobacco Survey – GATS) (English). Kyiv; 2017. Ukrainian.
Katelevska NM, Kosilova OYu, Volkov II. [Sodium glutamate as a dietary supplement and its health effects]. Doctor’s thesis. European Scientific Platform. 2019;2: 38-9.Ukrainian.
Zhang Y, Zhang L, Venkitasamy C, Pan Z, Ke H, Guo S, Wu D, Wu W, Zhao L. Potential effects of umami ingredients on human health: Pros and cons. Critical Reviews in Food Science and Nutrition. 2019;4: 9-1. DOI: https://doi.org/10.1080/10408398.2019.1633995
Bhattacharya T, Ghosh SK. Effect of neonatal exposure of Monosodium Glutamate in kidney of albino mice–a Histological study. Nepal Medical College Journal. 2019;21(2): 134-41. DOI: https://doi.org/10.3126/nmcj.v21i2.25113
Kucherenko DY, Kucherenko IS, Soldatkin OO, Soldatkin AP. Application of glutamate-sensitive biosensor for analysis of foodstuff. Biotechnologia Acta. 2018;11(4): 57-67. DOI: https://doi.org/10.15407/biotech11.04.057
Kayode OT, Rotimi DE, Kayode AA, Olaolu TD, Adeyemi OS. Monosodium glutamate (MSG)-induced male reproductive dysfunction: A mini review. Toxics. 2020;8(1): 7. DOI: https://doi.org/10.3390/toxics8010007
Umukoro S, Oluwole GO, Olamijowon HE, Omogbiya AI, Eduviere AT. Effect of monosodium glutamate on behavioral phenotypes, biomarkers of oxidative stress in brain tissues and liver enzymes in mice. World Journal of Neuroscience. 2015;5: 339-49. DOI: https://doi.org/10.4236/wjns.2015.55033
Manal Said T, Nawal AB. Adverse effects of monosodium glutamate on liver and kidney functions in adult rats and potential protective effect of vitamins C and E. Food and Nutrition Sciences. 2012;3: 651-9. DOI: https://doi.org/10.4236/fns.2012.35089
El-Demerdash FM, Tousson EM, Kurzepa J, Habib SL. Xenobiotics, oxidative stress, and antioxidants. Oxidative Medicine and Cellular Longevity. 2018; 2018: 1-2. DOI: https://doi.org/10.1155/2018/9758951
Rutska AV, Krynytska IY, Marushchak MI. [Indicators of free radical oxidation in rats under the condition of "passive smoking" against the background of long-term introduction of monosodium glutamate in sexual and age aspects]. Med i kiln khim. 2017;19(4): 115-22. Ukrainian.
Rutska AV. [Investigation of the enzyme link of the antioxidant system in rats under the influence of tobacco smoke on the background of the use of monosodium glutamate in sexual and age aspects]. Med i kiln khim. 2018;13(3): 145-53. Ukrainian.
Ashcheulova TV, Zaika MV, Gerasimchuk NN. [The relationship of immune activation and oxidative stress with the progression of arterial hypertension]. Ukr terap zhurn. 2007;2: 16-2. Ukrainian.
Ak-Akras R.K.M. The effect of oxidative stress on the level of cytokines in cell culture. Mezhdunar. nauch-islled zhurn. 2014;4(23): 78-80. Russian.
Lizurchik LV, Sheida EV. [The effect of oxidative stress on the level of cytokines in cell culture]. Vestn. OGU. 2014;6(167): 71-4. Russian.
Santiago HA, Zamarioli A, Neto MD, Volpon JB. Exposure to secondhand smoke impairs fracture healing in rats. Clinical Orthopaedics and Related Research. 2017;475(3): 894-902. DOI: https://doi.org/10.1007/s11999-016-5184-6
Falaleyeva TM, Samonina GE, Beregovaya TV, Dzyubenko NV, Andreyeva LA. [The effect of glyprolins on the structural – functional state of the gastric mucosa and rat body weight under conditions of prolonged administration of sodium glutamate]. Fizika zhivogo. 2010;18(1): 154-9. Russian.
d’Europa C. European Convention for the protection of Vertebrate Animal Used for experimental and other Scientific Purposes. 1986;123: 1-11.
Lykhatskyy PH, Fira LS. [Development of nitrooxidative stress and inflammatory processes in rats of different ages affected by tobacco smoke]. Svit medytsyny ta biolohii. 2017;4(62): 145-49. Ukrainian. DOI: https://doi.org/10.26724/2079-8334-2017-4-62-145-149
Orlova VD, Khrenov OA. [Influence of long-term smoking on LPS-induced synthesis of proinflammatory cytokines TNF-A and IL-1B by mononuclear leukocytes in patients with chronic pulmonary and chronic heart failure]. Zdobutky klinichnoi i eksperymentalnoi medytsyny. 2012;(1): 108-10. Ukrainian.
Nenasheva NM. [Bronchial asthma and smoking]. Effektivnaya farmakoterapiya. 2013;(10): 4-14. Russian. DOI: https://doi.org/10.36691/RJA625
Barbieri SS, Zacchi E, Amadio P, Gianellini S, Mussoni L, Weksler BB, Tremoli E. Cytokines present in smokers' serum interact with smoke components to enhance endothelial dysfunction. Cardiovascular Research. 2011; 90(3): 475-83. DOI: https://doi.org/10.1093/cvr/cvr032
Hart K, Haugen A, Zienolddiny S. Allele-specific induction of IL1B− 31 T/C promoter polymorphism by lung carcinogens. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2008;656: 8-14. DOI: https://doi.org/10.1016/j.mrgentox.2008.06.013
Hou L, Huang J, Lu X, Wang L, Fan Z, Gu D. Polymorphisms of tumor necrosis factor alpha gene and coronary heart disease in a Chinese Han population: interaction with cigarette smoking. Thrombosis Research. 2009;123(6): 822-6. DOI: https://doi.org/10.1016/j.thromres.2008.07.016
Yanbaeva DG, Dentener MA, Creutzberg EC, Wesseling G, Wouters EF. Systemic effects of smoking. Chest. 2007;131(5): 1557-66. DOI: https://doi.org/10.1378/chest.06-2179
Asfandiyarova NS. [Nicotine and the immune system]. Immunopatologiya, Allergologiya, Infektologiya. 2018;3: 12-6. Russian.
Asmolov OK, Rybak TA, Smolska IM, Herasymova NA, Baburina OA, Asmolov AK, Rybak TA, Smolʹskaya YN, Baburyna EA. [Influence of smoking on the pathogenesis of chronic obstructive pulmonary disease]. Odes med zhurn . 2008;6(110): 70-2. Ukrainian.
Rudyk MP, Pozur VV, Opeyda YV, Voyeykova DO, Khranovsʹka NM, Fedorchuk OH, Berehova TV, Ostapchenko LI. [Modulatory effects of sodium glutamate on circulating phagocytic cell functions in rats in vivo and in vitro]. Dopovidi Natsionalnoi akad nauk Ukrainy. 2017;5: 97-89. Ukrainian. DOI: https://doi.org/10.15407/dopovidi2017.05.089
Shapiro H, Lutaty A, Ariel A. Macrophages, meta-inflammation, and immuno-metabolism. The Scientific World Journal. 2011;11: 2509-29. DOI: https://doi.org/10.1100/2011/397971
Golovach IYU. [Nuclear factor κB (NF-κB) as an important pathogenetic factor and a new target in the treatment of rheumatic diseases]. Ratsionalnaya farmakoterapiya. 2012;3(24): 51-46. Russian.
Xu L, Sun J, Lu R, Ji Q, Xu JG. Effect of glutamate on inflammatory responses of intestine and brain after focal cerebral ischemia. World Journal of Gastroenterology: WJG. 2005;11(5): 733-36. DOI: https://doi.org/10.3748/wjg.v11.i5.733
Butcher BA, Kim L, Johnson PF, Denkers EY. Toxoplasma gondii tachyzoites inhibit proinflammatory cytokine induction in infected macrophages by preventing nuclear translocation of the transcription factor NF-κB. The Journal of Immunology. 2001;167(4): 2193-201. DOI: https://doi.org/10.4049/jimmunol.167.4.2193
Schölzke MN, Potrovita I, Subramaniam S, Prinz S, Schwaninger M. Glutamate activates NF-κB through calpain in neurons. European Journal of Neuroscience. 2003;18: 3305-10. DOI: https://doi.org/10.1111/j.1460-9568.2003.03079.x
Downloads
Published
How to Cite
Issue
Section
Accepted 2020-04-02
Published 2020-05-22
