PROGNOSTIC IMPORTANCE OF THE DYNAMIC OF THE MALONDIALDEHYDE IN RAT KIDNEY WITHIN ISCHEMIC REPERFUSION LIMIT DAMAGE
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2019.2.10695Keywords:
ischemic-reperfusion syndrome, kidney, lipoperoxidation, blood loss, tourniquetAbstract
The aim of the work. To establish the dynamic of the activity of TBA-active derivatives of lipid peroxidation (POL) – malonydialdehyde (MDA) in the rat kidney on the background of ischemic-reperfusion limb injury (IRI), which was caused by the experimental use of haemostatic tourniquet.
Materials and Methods. 260 non-linear white male rats with a body weight of 220–240 g were object of the experiment. The animals of the control group were removed from the study without surgery, and the experimental animals were divided into 5 groups (10 individuals each) – compared the animals with isolated overlapping of the hemostatic tourniquet, isolated blood loss and mechanical trauma with the animals in which the use was combined tourniquet with blood loss and tourniquet with mechanical injury. Animals were removed from the experiment after 1 hour, at 1; 3; 7 and 14 days after interventions by total blood flow from the heart.
Results and Discussion. In all study groups, increased activity of lipid peroxidation was found, with MDA in the group with isolated blood loss most actively increasing, and even more in the group with blood loss, which was combined with the use of a tourniquet.
Conclusions. The obtained results confirmed that the chemical hypoxia is capable of triggering lipid peroxidation, but the use of hemostatic tourniquet, causing the development of a typical ischemic-reperfusion syndrome, significantly complicates the course of the traumatic period; develop vascular and tissue disorders, which against the background of tension detoxification function of the liver cause involvement in the pathological process and the kidney.
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