FEATURES OF LIPID PEROXIDATION IN THE BLOOD AND HOMOGENATES OF RAT TISSUES IN CASE OF COMBINED INFLUENCE ОF STRESS FACTORS
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2019.2.10692Keywords:
stress factors, combination, TBA-active products, experimentAbstract
The aim of the work. To analyze the level of active products of thiobarbituric acid in blood and homogenate of rat tissues under the condition of stress factors and their combination..
Materials and Methods. The experiments were conducted on 54 white non-linear male rats weighing 200–220 g. Animals were divided into 5 groups: 1 – control group (n = 6), 2 – chronic exposure to tobacco smoke (n = 12), 3 – chronic black leaf tea intoxication (n = 12), 4 – chronic immobilization stress (n = 12), 5 – combined influence of stress factors (n = 12). The principle of determining the content of TBA-active products (TBA-AP) is based on the fact that at high temperature in acidic medium malonic dialdehyde reacts with thiobarbituric acid, forming a trimethin complex.
Results and Discussion. Chronic exposure to tobacco smoke is accompanied by activation of lipid peroxidation, in particular, the maximum changes in the content of TBA-AP are observed in the lung homogenate (3.5 times), in chronic intoxication with black tea – in the myocardial homogenate (3.5 times), with immobility – in the stomach homogenate (2.9 times) relative to the control group. With the combined effect of stressors, more pronounced changes are observed relative to the isolated effect of each factor: in the heart (6.9 times), in the lungs (6.3 times), in the stomach (6.6 times) and in periodontium (in 5.1 times) relative to the control group.
Conclusions. The combined effect of stressors causes the activation of free radical oxidation processes, which is characterized by changes in TBA reactants, in particular, more pronounced changes are observed relative to the isolated effect of each factor relative to the control group.
References
Treumova SI, Burmak YG, Petrov EE, Tribrat TA, Shut SV. [Adaptive and damaging mechanisms of stress development]. Visn probl biol i med. 2017;4(1): 74-8. Ukrainian.
Lysenko HI, Yashchenko OB, Mayatka OB. [Cardiovascular disorders in family physician practice]. Mystetstvo likuv. 2005; 71-5. Ukrainian.
Palii IH, Zaika SV, Vihristiuk HI. [Stress as a factor of occurrence of widespread therapeutic diseases and ways of its optimal correction]. Liky Ukr. 2009;7(133): 65-70. Ukrainian.
Maksiv KhYa, Marushchak MI. [Comborbidity of chronic obstructive lung disease with arterial hypertension: the state of the antioxidant defense system]. Visn med i boil doslidzh. 2019;1: 35-40. Ukrainian.
World Health Organization. WHO Report on the Global Tobacco Epidemic, 2008: The Mpower Package. Geneva. Switzerland: World Health Organization; 2008.
United States Departament of Health and Human Services: The Health Consequences of Smoking: Nicotine Addiction (A. Report of the Surgeon General) Rockville. Office on Smoking and Health;1998.
Picas AB. [Smoking cigarettes among the population and its role in the development of diseases]. Visn probl biol i med. 2016;1(126): 48-52. Ukrainian.
Shvets OV. [The benefits and risks of caffeine and caffeine-containing beverages]. Med transport Ukr. 2010;3: 91-96. Ukrainian.
Hensitskyi BI, Moiseenko OS. [Case of death due to toxic action of caffeine]. Topical issues of theoretical medicine. Topical issues of clinical medicine. Trace elements in clinical medicine. materials of scientific and practical conferences of students, young scientists, doctors and teachers (Sumy, April 21-23, 2010 ). Answer by LNPrystupa. Sumi: Sumy State University. 2010; 51. Ukrainian.
European convention to the protection of vertebrate animals used for eхperimental and other scientific purposes. Council of Europe. Strasbourg. 1986.
Santiago HA, Zamarioli A, Sousa Neto, Volpon JB. Exposure to secondhand smoke impairs fracture healing in rats. Clin Orthop Relat Res. 2017;475(3): 894-902. DOI: https://doi.org/10.1007/s11999-016-5184-6
Wei H, Zhang X, Zhao JF, Wang ZY, Bickers D, Lebwohl M. Scavenging of hydrogen peroxide and inhibition of ultraviolet light-induced oxidative DNA damage by aqueous extracts from green and black teas. Free Radic Biol Med. 1999;26(11-12): 1427-35. DOI: https://doi.org/10.1016/S0891-5849(99)00005-2
OECD. Guideline for testing of chemicals: acute oral toxicity – acute toxic Class Method. Paris, France; 2001.
Nikolaev OV, Kovaltsov MV, Yevtushenko TG. [Method for simulating chronic immobilization stress]. 2013 U 03868, published. 25.07.2013. Bul.14: 4. Stalemate. 82414 UA, A61D 99/00, G09B 23/28. Ukrainian.
Harutyunyan AB, Dubinina EE, Zybina NN. Methods for assessing free radical oxidation and the antioxidant system of the body. Methodical recommendations. [Методы оценки свободнорадикального окисления и антиоксидантной системы организма: метод. рекоменд] St. Petersburg. 2000. Russian.
Hnatiuk VV, Kononenko NM. [Gender and age-specific features of radical radical oxidation and antioxidant seizures during desynchronosis]. Visn Vinnyts nats med univer. 2014;2(18): 363-6. Ukrainian.
Kuryk LM. [Physical and chemical aspects of singlet-sour therapy in case of pathological processes]. Ukr. pulmon. zhurn. 2006;1: 66-8. Ukrainian.
Dubinska HM, Iziumska OM, Minakh OM. [Indicators of lipid peroxidation, antioxidant system and immunity in healthy people and people with increased susceptibility to respiratory tract infections]. Lab. diahnost. 2005;2: 18-21. Ukrainian.
Lemko OI. [Some aspects of etiology, pathogenesis and course of chronic obstructive pulmonary disease (Part I)]. Uzhhorod univer nauk visn, "Medytsyna". 2012;1(43): 180-9.
