PECULIARITIES OF THE ANTI-OXIDANT DEFENSE SYSTEM OF RATS DURING CARRAGEENAN INFLUENCE IN STREPTOZOTOCIN DIABETES
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2019.2.10690Keywords:
streptozotocin diabetes, chronic enterocolitis, superoxide dismutase, catalaseAbstract
The aim of the work. To determine the activity of the antioxidant protection system when using 1.0 % solution of κ-carrageenan in rats with streptozotocin-induced diabetes (SID).
Materials and Methods. The study was conducted on 120 white non-linear male rats complied with the principles of the European Convention for the Protection of Laboratory Animals. Diabetes mellitus was modeled by single intraperitoneal administration of streptozotocin to animals (Sigma Aldrich, USA, at a dose of 60 mg/kg body weight). Chronic enterocolitis (CEC) was reproduced by free animal access to 1.0 % solution of carrageenan in drinking water for 1 month.
Results and Discussion. It was found reduced enzyme activity of the antioxidant protection system, in particular, SOD activity in erythrocyte hemolysate decreased by 37.8 % and catalase in blood plasma by 48.5% in animals with SID. Experimental CEC was accompanied by an increase in the activity of the enzyme link of the antioxidant protection system (SOD by 44.8 % and catalase by 25.6 %, p<0.01) vs. control. In rats with chronic enterocolitis on the background of streptozotocin diabetes decreased activity of the enzyme link of the antioxidant system, in particular, the activity of SOD in hemolysate of erythrocytes decreased by 59.5 % and catalase in blood plasma – by 62.7 %.
Conclusions. In case of chronic enterocolitis on the background of streptozotocin-induced diabetes, oxidative processes are activated by reducing the superoxide dismutase and catalase activity in the rats’ blood.
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