PECULIARITIES OF THE CHANGE IN THE INDICATORS OF PROINFLAMMATORY CYTOKINES IN RATS WITH MODELED GALACTOSAMINE HEPATITIS ON THE BACKGROUND OF MERCAZOLIL-INDUCED HYPOTHYROIDISM
DOI:
https://doi.org/10.11603/bmbr.2706-6290.2019.1.10513Keywords:
galactosamine hepatitis, hypothyroidism, cytokines, IFN-γ, IL-1α, IL-4, MCP, TNF-αAbstract
The aim of the work. To investigate changes in cytokine profile and functional status of endothelium in rats for mercazolil-induced hypothyroidism with concomitant galactosamine hepatitis.
Materials and Methods. The studies were performed on white male rats weighing 180–200 g obtained from vivarium of I. Horbachevsky Ternopil National Medical University. Hypothyroidism was modeled by daily administration of per os using a special probe of mercazolil pharmacopoeial thyrostatics at a dose of 25 mg/kg for 21 days. The effect of hypothyroidism on the course of the inflammatory process in hepatitis was studied in a model of galactosamine hepatitis (GAH), which was caused by intraperitoneal administration to experimental animals galactosamine at a dose of 400 mg/kg in the form of a 20 % solution.
Results and Discussion. It was found that the TNF-α index in animals with simulated hypothyroidism increased by 1.05 times, IL 1-α – by 1.41 times, IL-4 – 1.33 times, INF-γ – 5.56 times relative to intact animals. Moreover, monocyte chemoattractant protein (MCP) decreased by 0.95 times. After simulated galactosamine hepatitis, it was found that TNF-α increased by 1.13 times, IL-1α – 1.49 times, IL-4 – 1.56 times, INF-γ – 6.57 times MCP – 1.06 times relatively intact animals. Following the simulated galactosamine hepatitis, the following changes occurred on the background of hypothyroidism: TNF-α increased 1.32-fold, IL-1α – 1.70-fold, IL-4 – 1.94-fold, INF-γ – 5.89-fold MCP – 1.11 times relative to intact animals.
Conclusions. It is concluded that under the conditions of experimental galactosamine hepatitis, hypothyroidism overproduces interproduction of interleukin-1α (IL-1α), interleukin-4 (IL-4), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), as well as hypoproduction of monocytic chemoattractant protein (MCP), which can cause significant changes in liver tissues.
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Accepted 2019-11-14
Published 2019-11-14
