Investigation of peroxidase preparation composition and properties isolated from horseradish roots
DOI:
https://doi.org/10.11603/mcch.2410-681X.2016.v0.i2.6675Keywords:
horseradish peroxidase, electrophoresis, biochemical properties.Abstract
Using the precipitation of proteins by zinc and ammonium sulfates, then by polymer and subsequent dialysis, the peroxidase preparation from horseradish roots was isolated (RZ=0.7), with yield by protein 1.7 % and specific peroxidative activity 7.6 U. By SDS-electrophoresis method it was verified the obtaining of partially purified peroxidase preparation with molecular mass (43±0.5) kDa. By the native electrophoresis method it was determined, that 80.8 % of the total preparation protein possesses expressed peroxidase activity.
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