CHANGES OF LIPID PEROXIDATION AND ANTIOXIDANT DEFENSE OF HIGH AND LOW-RESISTANCE TO ACUTE HYPOXIC HYPOXIA RATS OF DIFFERENT SEX IN IMMOBILIZATIONAL STRESS

Authors

  • Iu. M. Ordynskyi I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • O. V. Denefil I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2019.v.i2.10302

Keywords:

stress, rats, resistance to hypoxia, lipid peroxidation, antioxidant system

Abstract

Introduction. Determining the mechanisms of damaging the influence of stress on the heart in persons with different reactivity can contribute to the development of individual methods of correction.

The aim of the study – to determine the effect of immobilization stress on changes in peroxide lipid oxidation and antioxidant defense in rats of different sex with high and low resistance to hypoxia (HR, LR).

Research Methods. Stress was modeled 4 times by an hour immobilization of rats on a back down with an interval of 24 hours. In the heart, the diene and triaene conjugates, schiff bases, TBA-active products, superoxide dismutase (SOD), catalase activity in heart and ceruloplasmine and activity of peroxides were determined.

Results and Discussion. In control HR males, in comparison with LR, lower activity of lipid peroxidation, higher activity of SOD, concentration of ceruloplasmine, blood peroxides were observed; in HR-females, in comparison with LR, more diene and triene conjugates, schiff bases, SOD, ceruloplasmine and activity of peroxides; less TBA‑active products and lower catalase activity. Immobilization led to an increase in all investigated products of lipid peroxidation (in all groups of animals the TBA-active products increased the most intensively). The schiff bases also significantly increased in females. This indicates the development of oxidative stress, which is more pronounced in males than in females. In females, lipid peroxidation products metabolize faster, than in males. At the same time, the antioxidant defense system was activated: the superoxide dismutase activity (more in low resistance to hypoxia animals) and catalase activity (more in high resistance to hypoxia individuals) increased maximally in the females, and in males, the concentration of ceruloplasmin (most notably in low resistance to hypoxia animals). The peroxidase activity of blood decreased in all observed groups of animals.

Conclusions. Immobilization of rats leads to oxidative stress in the heart, but the mechanisms of their development, adaptation and compensation depend on resistance to hypoxia and sex of animals.

References

Crea, F., Battipaglia, I., & Andreotti, F. (2015). Sex differences in mechanisms, presentation and management of ischaemic heart disease. Atherosclerosis, 241 (1), 157-168.

Kötter, T., & Niebuhr, F. (2016). Resource-oriented coaching for reduction of examination-related stress in medical students: an exploratory randomized controlled trial. Adv. Med. Educ. Pract., 7, 497-504.

Rodin, R., Bonanno, G.A., Rahman, N., Kouri, N.A., Bryant, R.A., Marmar, C.R., & Brown, A.D. (2016). Expressive flexibility in combat veterans with posttraumatic stress disorder and depression. J. Affect. Disord., 207, 236-241.

Pimple, P., Shah, A.J., Rooks, C., Bremner, J.D., Nye, J., Ibeanu, I., Raggi P., & Vaccarino, V. (2015). Angina and mental stress-induced myocardial ischemia. J. Psychosom. Res., 78 (5), 433-437.

Berezovskyy, V.A. (1978). Gipoksiya i individualnye osobennosti reaktivnosti [Hypoxia and individual particularities of reactivity]. Kyiv: Naukova dumka [in Russian].

Kulynskyy, V.I., & Olkhovskyy, I.A. (1992). Dve adaptatsionnye strategii v neblagopriyatnykh usloviyakh: rezistentnaya i tolerantnaya. Rol gormonov i retseptorov [Two adaptation strategies in adverse conditions: resistant and tolerant. The role of hormones and receptors]. Uspekhi sovremennoy biologii – Advances of Modern Bio­logy, 112, 697-711 [in Russian].

Khyshiktuev, B.S., Khyshiktueva, N.A., & Iva­nov, V.N. (1996). Metody opredeleniya produktov pe­rekisnogo okisleniya lipidov v kondensate vydykhayemogo vozdukha i ikh klinicheskoye znacheniye [Methods of determination of products of lipids peroxidation in exhaled air condensate and their clinical significance]. Klini­cheskaya laboratornaya diagnostika – Clinical Laboratory Diagnostics, 3, 13-15 [in Russian].

Stefanov, O.V. (Ed.). (2001). Doklinichni do­slidzhennia likarskykh zasobiv: metodychni rekomendatsii [Preclinical research of medicinal products: methodical recommendations]. Kyiv: Avitsenna [in Ukrainian].

Chevary, S., Chaba, Y., & Sokey, Y. (1985). Rol superoksiddysmutazy v okislitelnykh protsessakh kletki i metod opredeleniya ee v biologicheskikh materialakh [The role of superoxide dismutase in the oxidative processes of the cell and the method for its determination in biological materials]. Laboratornoye Delo – Laboratory Work, 11, 678-681 [in Russian].

Korolyuk, M.A., Ivanova. L.I., Mayorova, I.G., & Tokarev, V.E. (1988). Metod opredeleniya aktivnosti katalazy [Method for determination of catalase activity]. Laboratornoye delo – Laboratory Work, 1, 16-19 [in Russian].

Zabolotko, V.М. (Ed). (2003). Klinichna ta laboratorna diahnostyka. Normatyvni, dyrektyvni, pravovi dokumenty: zbirnyk: v 2 ch. [Clinical and laboratory diag­nostic. Norm, directive, low documents: in 2 parts]. Кyiv: МEC “Мedinform” [in Ukrainian].

Popov, Т., & Neykovska, L. (1971). Metod opredeleniya peroxidaznoy aktivnosti krovi [Method for determination of blood peroxides activity]. Gigiyena i sanitariya – Hygiene and Sanitary, 10, 89-93 [in Russian].

Morozova, V.S. (2013). Funktsionuvannia antyoksydantnoi systemy miokardu shchuriv za umov shtuchnoho hipobiozu [Functioning of the antioxidant system of myocardium of rats under conditions of artificial gipobiosis]. Visnyk problem biolohii i medytsyny – Bulletin of Biological and Medical Problems, 2 (103), 3, 86-90 [in Ukrainian].

Published

2019-07-11

How to Cite

Ordynskyi, I. M., & Denefil, O. V. (2019). CHANGES OF LIPID PEROXIDATION AND ANTIOXIDANT DEFENSE OF HIGH AND LOW-RESISTANCE TO ACUTE HYPOXIC HYPOXIA RATS OF DIFFERENT SEX IN IMMOBILIZATIONAL STRESS. Medical and Clinical Chemistry, (2), 103–109. https://doi.org/10.11603/mcch.2410-681X.2019.v.i2.10302

Issue

Section

ORIGINAL INVESTIGATIONS