Reactive arthritis imunopathogenesis
DOI:
https://doi.org/10.11603/mcch.2410-681X.2017.v0.i3.8195Keywords:
reactive arthritis, lymphocytes, immunological parameters, infection.Abstract
Introduction. It is known, that reactive arthritis is one of the widespread joints pathology. In recent years an increase in the number of patients with this pathology has been observed. Causative factors of reactive arthritis are completely unexplored today, the question of pathogenesis of reactive arthritis remains for discussions. Inflamatory diseases of joins, wich are developing due to immune disorders after urogenital, intestinal or respiratory infection belong to reactive arthritis.
The aim of the study – to learn the functional activity of cells of phagocytic system (monocytes and neutrophils of peripheral blood) and detection of diagnostic value of phagocytic reactions in cases of reactive arthritis.
Research Methods. The investigations were conducted on blood samples, of patients with reactive arthritis (n=20) and healthy donors (n=12). The phagocytic activity of neutrophils was determined using method based on endocytosis latex particles by phagocytes, which are visualized in cytoplasm of cells in form of round granules with blue color. Test with nitro blue tetrazolium (NBT-test) was carried out according to the change of color NBT in presents of reactive oxygen species. The counting of neutrophils with blue color granules allows to determine fraction of neutrophils with reactive oxygen species.
Results and Discussion. Trigger factor causing development of reactive arthritis mostly is Chlamidia trachomatis (36 %). Patients with this pathology have statistically significant changes of functional metabolic activity of neutrophils and monocytes of blood. By such indicators as amount of white blood cells, monocytes and neutrophils in peripheral blood and phagocytic activity in cases of reactive arthritis signs of inflammation have been detected.
Conclusion. Received data testify about decreased phagocytic activity of neutrophils and monocytes of blood with the development of reactive arthritis.
References
Dzhus, M.B. (2010). Kliniko-imunolohichni osoblyvosti perebihu reaktyvnoho artrytu [Clinical and immunological features of the course of reactive arthritis]. Ukr. revmatol. zhurn. – Ukrainian Rhematological Journal, 17 (3), 372 [in Ukrainian].
Lapovets, L. & Kutsyk, B. (2004). Laboratorna imunolohiia [Laboratory immunology]. Kyiv: Aral [in Ukrainian].
Nazarenko, G.I., & Kishkun, A.A. (2000). Klinicheskaya otsenka rezultatov laboratornykh issledovaniy [Clinical evaluation of laboratory research results]. Moscow: Meditsina [in Russian].
Spaska, H.O. (2011). Reaktyvnyi artryt: suchasnyi pohliad na problemu [Reactive arthritis: modern look on the problem]. Ukr. med. chasopys – Ukrainian Medical Journal, 11-12 (6), 55-59 [in Ukrainian].
Ajene, A.N., Walker, C.L., & Black, R.E. (2013). Enteric pathogenes and reactive arthritis: a systematic review of Campilobacter, Salmonella and Shigella-associated reactive arthritis. J. Health Popul. Nutr., 31 (3), 299-307.
Аppel H., Kuon W., & Kuhne M. (2003). Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in chlamidia-triggred reactive arthritis. Arthr. Res. Ther., 6 (6), 521-534.
Bas, S., Kvien, T.K., & Buchs, N. (2013). Lower level of synovial fluid interferon-gamma in HLA-B27-positive than in HLA-B27-negative patients with Chlamydia trachomatis reactive arthritis. Rheumatology (Oxford), 42, 461-467.
Cargnelutti, E., & Di Genaro, M.S. (2013). Reactive arthritis: from clinical feayures to pathogenesis. Intern. J. Clin. Med., 4, 20-30.
Carter, J.D. (2010). Treating reactive arthritis: insights for the clinican. Ter. Adv. Muskoloskelet. Dis., 2 (1), 45-54.
Carter, J.D., & Hudson, A.P. (2009). Reactive arthritis: clinical aspects and medical management. Rheum. Dis. Clin. North. Am., 35 (1), 21-44.
Сolmegna, I., Cuchacovich, R., & Espinoza, L.R. (2004). HLA-B27-associated reactive arthritis: pathogenetic and clinical considerations. Clin. Microbiol. Rev., 17, 348-369.
Helmick, C.C., Felson, D.T., & Lawrence, R.C. (2008). Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Arthritis & Rheumatism, 58 (1), 15-25.
Kim, P.S., Klausmeier, T.L., & Orr, D.R. (2009). Reactive arthritis: a review. J. Adolesc. Health, 44 (4), 309-315.
Кwiatkowska, В., Filipowicz, A., & Sosnowska, A. (2009). Reactive arthritis. Polish Archves, 11 (11), 98-104.
Schumacher, H.R. (2002). Chlamidial arthritis. Proc. Meat. Eur. Soc. Chlam. Res., 3, 36-39.
Stavropoulos, P.G., Soura, E., Kanelleas, A. (2015). Reactive arthritis. J. Eur. Acad. Dermatol. and Venereol., 29 (3), 415-424.
Toivanen, A., & Toivanen, P. (2001). Reactive arthritis. Isr. Med. Assoc. J., 681-685.
