INDEXES OF ENERGY METABOLISM IN HEART TISSUE OF EXPERIMENTAL ANIMALS UNDER CONDITIONS OF THE INFLUENCE OF CADMIUM CHLORIDE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v0.i1.9900Keywords:
energy metabolism, cadmium chloride, Na , K , Mg2 - ATPase, adenosine triphosphoric acid, glucose, lactate dehydrogenaseAbstract
Introduction. Cadmium chloride can enter the body of humans and animals with food, drinking water and atmospheric air. Their effect is to suppress the functional state of mitochondria, which leads to the depletion of energy resources and the violation of a number of vital processes. Cadmium, penetrating into cells, interacts with mercaptogroups, suppresses enzyme systems of energy supply, by replacing bivalent metals that are part of enzymes.
The aim of the study – to determine the state of energy metabolism in the cardiac muscle of experimental animals (rats) under conditions of cadmium chloride.
Research Methods. Intoxication was carried out by intravenous administration of cadmium chloride at a dose of 1/10 LD50 for 10 days. Animals were divided into two groups: intact and experimental, which were injected with cadmium chloride. The material was collected after decapitation under tiopental anesthesia at 1st, 14th, 28th day after the completion of the administration of the toxicant. Indicators of energy metabolism were determined as follows: the activity of ATPase, lactate dehydrogenase was determined by the enzymatic method; concentration of glucose – glucoxidase method, the level of pyruvate, lactate and adenosine triphosphate (ATP) was determined spectrophotometrically. The content of macro- and micronutrients (Cd, Zn, Cu, Mg) was determined using an atomic absorption spectrophotometer.
Results and Discussion. The obtained results testify to the increased use of glucose by the cardiac muscle in anaerobic way, as there is an increase in the concentration of lactic acid. This is confirmed by the literature sources on the development of hypoxia under the conditions of cadmium ions. At that time, the level of adenosine triphosphoric acid decreases as well as the activity of ATPase in the heart tissue. We also observed the increase in the content of magnesium (the activator of ATPase) and zinc (the activator of lactate dehydrogenase) in the heart of experimental animals.
Conclusion. The conducted studies indicate a violation of the energy supply of tissue of the heart muscle for cadmium intoxication, which is confirmed by the activation of glucose anaerobic oxidation, a decrease in its level and the content of ATP in the myocardium.
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