SOME PARAMETERS OF THE PROTEIN METABOLISM IN RATS AFTER AMANITA-PHALLOIDES INTOXICATION

Abstract

Introduction. Amanita phalloides is one of the most dangerous poisonous fungi in the world. Toxins of poison amanita refer to strong poisons, and because of the absence of specific antidotes to them, the treatment of these poisons remains an important problem. The severe clinical picture of poisoning of Amanita phalloides is due to toxic lesions of the liver and kidneys, the development of acute gastroenterocolitis, the emergence of endotoxicosis, associated with violation of homeostasis and accumulation in the body of toxic substances of endogenous origin.

The aim of the study – to reseach the effect of the poison of Amanita phalloides on some parameters of protein metabolism in rats.

Research Methods. The studies were conducted on non-linear white 18–24-month male rats (old age). The animals were poisoned by a single intraperitoneal administration of an extract of Amanita phalloides obtained by H. Wieland in a dose of 85 mg/kg body weight. After 6, 24 and 72 hours after poisoning, the concentration of total protein and protein fractions, creatinine and urea in blood serum was determined. The state of endogenous intoxication was assessed by the erythrocytic index of intoxication and the content of medium massmolecules.

Results and Discussion. It has been established that poisoning of experimental animals with toxins of Amanita phalloides leads to significant disorders of protein metabolism. The most pronounced changes in the protein metabolism are observed after 72 hours from the moment of intoxication. In rats, there is a decrease in urea concentration, increased creatinine concentration, hypoproteinemia, hypoalbuminemia, a decrease in the content of α₁- and α₂-globulins and an increase in the β- and γ-globulin content have been observed. The most pronounced changes in the incidence of endogenous intoxication are characteristic after 24 hours from the moment of intoxication.

Conclusions. The obtained results testify about the marked negative effect of Аmanita phalloides on the protein synthesizing system of hepatocytes. It was defined, that the progression of the pathological process in the liver and the depletion of the compensatory capacity cause the increasing of endogenous intoxication indices.