EFFECT OF PHYTOCOMPOSITION ON THE GOAT'S-RUE AND HALEVIT ON METABOLIC CHANGES IN ANIMALS WITH DEXAMETHASONE-INDUCED INSULIN RESISTANCE

Abstract

Introduction. In Ukraine and in the world, type 2 diabetes mellitus is one of the most common chronic diseases characterized by metabolic disorders, insulin resistance and relative insufficiency of insulin. The main purpose of the treatment of this disease is to preserve the function of β-cells and reduce insulin resistance.

The aim of the study – to investigate the effect of the phytocomposite of the goat's-rue and Halevit on metabolic changes in insulin resistance rats, induced by dexamethasone.

Research Methods. The research object is the photocomposition of the dry extract Galega officinalis L, blueberries and taurine, as well as its liposomal form under the conditional name Halevit. The investigated photocompositions standardized for the content of flavonoids. Another medicine is Arfa combi.

The study was conducted on white rats. The prevalence level was assessed with changes in glycaemia levels after an oral glucose tolerance test, and the lipid metabolism status was assessed for changes in cholesterol by the value of the integral index of the area under the glycemic curve – AUCglu (glycemic area under curve, AUCglu, mmol / L × X), common lipids and triglycerides, the corresponding changes in the LP / AOS system were evaluated with the content of TBA-AP and G-SH.

Results and Discussion. Investigation of metabolite-tropic effects of phytocompositions on the models of insulin resistance induced by dexamethasone showed that the application of Halevit also leads to more effective improvement of practically all investigated parameters of carbohydrate and lipid metabolism, balance of parameters of oxidant-antioxidant system as compared to the equivalent ones in the group of animals treated by reference phytopreparation Arfa combi and goat's-rue phytocomposition. This herbal remedy more effectively corrected the level of glycemia at OGTT in animals with insulin resistance. At the same time, the dynamics of glycemia at glucose challenge in those animals was not significantly different from those of the control group.

In animals with insulin resistance there was a pronounced dyslipidemia characterized by an increased level of total lipids, cholesterol and triglycerides, which may be due to the activation of lipolysis in adipose tissue and disorderof of lipids utilization in liver. Investigated phytopreparations in experimental insulin resistance caused a distinct hypolipidemic and hypocholesteremic activity.

Conclusions. Under conditions of experimental dexamethasone-induced insulin resistance in animals, the photocompositions tested are capable of preventing carbohydrate and lipid metabolism disorders, correcting the disturbances of the oxidant-antioxidant balance in the tissues of the body by preserving the natural reserves of the intracellular antioxidant – reduced glutathione, which contributes to reducing the manifestations of insulin resistance and glucose tolerance in OTTG.