STATE OF ERYTHROCYTE ADENYLATE SYSTEM IN FEMALE RATS UNDER THE INFLUENCE OF TESTOSTERONE AND ESTRADIOL UNDER CONDITIONS OF AN EXPERIMENTAL OVERACTIVE BLADDER

Abstract

Introduction. The prevalence of the symptom complex of overactive bladder, especially among women before and after menopausal age, the complexity of its treatment is due to uncertainty until the end of the mechanisms of its development. Application to clinical practice of hormonal drugs requires pathogenetic justification. These processes require a unified approach to their refinement, which dictates the need to create an experimental model with the study of the adenyl system.

The aim of the study – to learn the change of quantitative indices of erythrocyte adenylate system in female rats with experimental overactive bladder syndrome (OBS) corrected with testosterone and estradiol.

Research Methods. The experiments were conducted on 50 female rats of the Wistar line which were divided on 4 groups. The group 1 consisted of intact animals (n = 10). Animals of the group 2 (n = 10) with experimental overactive bladder were not subjected to pharmacological correction. In the group 3 of animals (n = 20), overactive bladder was modeled and two subgroups were organized, corrected with testosterone and estradiol, respectively. The animals of the group 4 (n = 10) were administered with a composition of testosterone and estradiol. Sex hormones were administered at doses of 1.0 mg/animal (testosterone), and 0.2 mg/animal (estradiol), depending on the average weight. The overactive bladder model was induced by daily intravenous administration of Homviotensin (Germany) at a dose of 0.45 mg/kg of body weight per active substance reserpine for 2 weeks. The concentration of adenyl nucleotides (ATP, ADP and AMP) and combination thereof in the blood was determined using the Agilent 1200 chromatograph (Agilent Technologies, USA).

Results and Discussion. We found a significant disturbance of the content of adenyl nucleotides in the overactive bladder animal model: a statistically important decrease in ATP and AMP levels at increased ADP, which contributed to a sharp reduction of the total content of the study nucleotides by 50.2 %. Under the influence of testosterone and estradiol the quantitative indices of ATP, ADP and AMP content in erythrocytes were restored, that suggested the protective effect of sex hormones on energy profile.

Conclusions. A sharp decrease of ATP and AMP levels and an increase of ADP level, which caused the dynamic fluctuations of energy charge and the energy cellular potential, was revealed in the overactive bladder of experimental animals. This suggests the development of a deficiency of high-energy phosphate compounds, which confirms a depletion of ATP-ADP-AMP adenylate system at overactive bladder. The androgen-estrogenic action at experimental overactive bladder syndrome demonstrates the pathogenetic orientation of protective effect on restoration of energy resources of cells, and, therefore, normalizing all components of the adenylate system of red blood cells, in particular ATP.