STUDY OF ANTIOXIDANT PROPERTIES OF EXTRACT FROM LEAVES OF SPINATE OF A COURSE ON A MODEL OF LIVER TETRACHLOROMETHANE

Abstract

Introduction. According to modern data, in Ukraine, 20–30 % of all liver diseases constitute its toxic lesions. The search for potential hepatoprotectors was carried out in recent years among a large number of medicinal substances of different origin and structure, however, natural antioxidants, mostly of plant origin, turned out to be the most promising because of their low cost and high level of safety.

The aim of the study – to investigate the antioxidant properties of a thick extract of garden spinach leaves in an experiment on rats affected by carbon tetrachloride.

Research Methods. The experiments were carried out on 60 white male rats, which simulated acute toxic liver damage by injecting carbon tetrachloride twice (every other day) in the form of a 50 % oil solution at a dose of 1.0 ml / kg of animal body weight. The object of the study was a thick extract of spinach leaves at a dose of 150 mg / kg body weight. Studies were conducted on the 4th, 7th and 10th day of the development of toxic hepatitis. The activity of oxidative processes and the state of the antioxidant system were assessed by the content of TBA – active products and 2,4 - dinitrophenylhydrazones, ceruloplasmin, catalase activity.

Results and Discussion. The defeat of rats with toxic doses of carbon tetrachloride was accompanied by the activation of lipid peroxidation and oxidative modification of proteins, which led to the development of oxidative stress in the body. Under these conditions, a decrease in the activity of the antioxidant system was observed, as indicated by the inhibition of catalase activity in the body of affected rats. Application for the purpose of correcting the identified violations of the dense extract from the leaves of garden spinach caused a decrease in the activated oxidative processes and the restoration of the protective-compensatory forces of the body, as indicated by the normalization of catalase activity and the content of ceruloplasmin. The studied extract was somewhat different in efficiency from the known silymarin hepatoprotector (Karsil), but at the end of the study its effect on oxidative stress indices differed little from the comparator drug.

Conclusion. The thick extract from the leaves of garden spinach under conditions of toxic liver damage exhibits hepatoprotective properties that are realized through the antioxidant effect. This leads to the expediency of further study of this medicinal product.