Role of oxidative stress in pathogenesis of chronic enterocolitis on the background of streptozotocin-induced diabetes
DOI:
https://doi.org/10.11603/mcch.2410-681X.2017.v0.i1.7414Keywords:
streptozotocin-induced diabetes, chronic enterocolitis, oxidative modification of proteins, experiment.Abstract
Introduction. In the medical literature there are a sufficient number of publications reporting on the relationship between inflammatory bowel disease, colorectal cancer and type 2 diabetes, together with inflammation and dysbiosis. Existing data suggest that, on the one hand, diabetes is an important factor in the pathogenesis of diseases of the intestine, and on the other hand, chronic bowel disease is associated with diabetes.
The aim of the study – to evaluate changes in oxidative processes in serum and small intestine tissues of rats with chronic enterocolitis on the background of streptozotocin-induced diabetes, in terms of oxidative modification of proteins.
Materials and Methods. The study was conducted on 106 white male nonlinear-rats. Diabetes mellitus (streptozotocin diabetes STD) was modeled by a single intraperitoneal streptozotocin administration. Chronic enterocolitis (ChEC) was modeled by free access to animals in 1.0 % carrageenan solution in water for 1 month. The degree of oxidative modification of proteins (OMP) was determined according to the formation of aldehyde and ketone groups during transfarmation 2.4 dinitrophenylhydrazine to the 2.4-dynitrofenilhidrazone.
Results and Discussion. The results of our research proved that neutral ketone derivatives content, recorded at a wavelength of 370 nm, exceeding reference values of 2 and 3 experimental groups by 7.5 % (p<0.05), and 4 – by 15.0 % (p<0.01). The level of alkaline oxidizing modified proteins in the serum, recorded at a wavelength of 430 nm, statistically significantly increased in the STD in 91.6 %, in the ChEC in 64.5 %, and during combined pathology – in 166.9 % (Table. 1). In the small intestine tissue concentration of OMP370 also increased in all experimental groups regarding control, particularly in groups 2 and3 in 26.8 % in the 4 – in 82.1 % (p <0.01). Content of alkaline aldehyde derivatives content, recorded at a wavelength of 430 nm, increased both in STD and ChEC with an average of 46.0 %, while in ChEC on the background of STD – in 151.4 %.
Conclusion. During chronic enterocolitis on the background of diabetes mellitus increases the oxidative modification of proteins, which is characterized by growth and aliphatic aldehyde and ketone derivatives with neutral and alkaline character in serum and small intestine tissues, with a predominance of protein fragmentation effects (p <0.05).
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