INVESTIGATION OF PHENOLIC COMPOUNDS IN THE RAW MATERIAL OF RUDBECKIA HIRTA L.

Abstract

Introduction. Rudbeckia hirta L. is an herbaceous plant of the Asteraceae family, traditionally used for the treatment of inflammatory diseases such as colds, ulcers, and snake bites. Rudbeckia hirta L. is utilized in traditional medicine in certain regions of North America. Extracts from the roots of Rudbeckia hirta L. are used for the treatment of gynecological diseases and sexually transmitted infections. Polysaccharides contained in Rudbeckia hirta L. exhibit immunomodulatory properties. The aim of the study – to determine the individual components of flavonoids and hydroxycinnamic acids in the herb of Rudbeckia hirta L. using the high-performance liquid chromatography (HPLC) method. Research Methods. The material for the study was the herb of Rudbeckia hirta L., harvested in the territory of the Chernivtsi region. Individual components of flavonoids and hydroxycinnamic acids were determined using the HPLC method on an Agilent Technologies 1200 liquid chromatograph. Results and Discussion. Using the HPLC method, eight individual components of flavonoids were identified, including rutin (1 308,96 μg/mg), isoquercetin (97,68 μg/mg), astragalin (43,93 μg/mg), naringin (673,82 μg/mg), fisetin (2 381,39 μg/mg), quercetin (800,9 μg/mg), apigenin (307,95 μg/mg), and baicalein (562,72 μg/mg). Among the individual components of hydroxycinnamic acids, the presence and quantitative content were determined for chlorogenic acid (7 992,58 μg/mg), p-coumaric acid (763,85 μg/mg), caffeic acid (191,45 μg/mg), syringic acid (210,95 μg/mg), trans-cinnamic acid (93,47 μg/mg), quinic acid (85,35 μg/mg), sinapic acid (15,11 μg/mg), and trans-ferulic acid (12,63 μg/mg). Conclusions. Individual components of phenolic compounds in the herb of Rudbeckia hirta L. were identified using the HPLC method. It was established that among the flavonoid components, fisetin (2 381,39 μg/mg) and rutin (1 308,96 μg/mg) had the highest content. Among the hydroxycinnamic acids, chlorogenic acid (7 992,58 μg/mg) had the highest content. The obtained results indicate the potential for further in-depth phytochemical studies of the biologically active substances in the herb of Rudbeckia hirta L.