DEVELOPMENT OF METHODS FOR THE ISOLATION OF PROPRANOLOL HYDROCHLORIDE FROM BIOLOGICAL MATERIAL

Abstract

Introduction. Diseases of the cardiovascular system (CVD) occupy one of the first places among diseases in the world. In Ukraine, 64 % of deaths are caused by cardiovascular diseases. Drugs of various groups are used in the treatment: beta-blockers, ACE inhibitors, diuretics, calcium antagonists, peripheral vasodilators and nitrates. Anaprilin was one of the first β-blockers discovered by scientists, so doctors have been prescribing it to their patients for a long time and know its advantages and disadvantages. It remains a kind of “benchmark” for evaluating the effectiveness and safety of new β-blockers under development.

The aim of the study – development of methods for isolating propranolol hydrochloride from biological material.

Research methods. During the study, propranolol hydrochloride was used, isolated from “Anaprilin” tablets, which contained 40 mg of propranolol in a tablet in the form of 38.6 mg of propranolol hydrochloride (the purity of the obtained substance was confirmed by TLC, UV spectrophotometry).

Results and Discussion. The UV spectra of the substance under study had light absorption maxima at a wavelength of 293±2, and the nature of light absorption coincided with the UV spectrum of a standard solution of propranolol in a 96 % ethanol solution. Quantification was done by the calibration graph equation y = 0.2161x – 0.0239, correlation coefficient (R₂) = 0.9997. The developed methods made it possible to isolate propranolol hydrochloride according to the method of О. O. Vasilieva 26.01±1.67 %, Stas – Otto – 46.04±2.24, according to the method of I. Sshezhinski – 67.22±3.16%.

Conclusion. Effective conditions for sample preparation of propranolol hydrochloride from biological material by UV-spectrophotometric determination were determined. The obtained results are of practical importance for the creation of an algorithm of toxicological research of biological objects for the presence of the specified substance in the case of fatal drug intoxications.