DYNAMICS OF THE ENZYME LINK PARAMETERS OF THE HEART’S ANTIOXIDANT DEFENSE UNDER CONDITIONS OF CRANIOSKELETAL TRAUMA IN RATS OF DIFFERENT SEXES AND THE EFFECTIVENESS OF 2-ETHYL-6-METHYL-3-HYDROXYPYRIDINE SUCCINATE CORRECTION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2024.i2.14764Keywords:
craniocerebral trauma, skeletal trauma, male rats, female rats, oxidative stress, heart, antioxidant defense, 2-ethyl-6-methyl-3-hydroxypyridine succinateAbstract
Introduction. Craniocerebral trauma is one of the leading causes of mortality and morbidity worldwide. This injury is often combined with damage to the bones of the skeleton and is more common in men than in women. Gender belongs to the biological attributes of a person and can definitely affect the course of a severe trauma and its systemic impact on the body.
The aim of the study – to determine the dynamics of the enzyme link parameters of the heart’s antioxidant defense under conditions of cranioskeletal trauma in rats of different sexes and the effectiveness of 2-ethyl-6-methyl-3-hydroxypyridine succinate correction.
Research Methods. The experiments were conducted on 156 white Wistar line rats of both sexes weighing 200–220 g. All rats were divided into 6 groups: 1 and 2 control groups, consisting of 6 female and 6 male rats; 1 and 2 experimental groups, consisting of 45 rats of each sex, which were modeled with cranioskeletal trauma; 3 and 4 experimental groups, consisting of 27 rats of each sex, which were corrected after the injury by intraperitoneal injection of 2-ethyl-6-methyl-3-hydroxypyridine succinate solution. Rats in experimental groups 1 and 2 were excluded from the experiment after 1, 3, 7, 14, and 28 days, and rats in experimental groups 3 and 4 were excluded after 7, 14, and 28 days. The heart was taken for the investigation, and superoxide dismutase and catalase activity was determined in the homogenate.
Results and Discussion. In the posttraumatic period, the dynamics of the investigated parameters in rats of different sexes was similar. Indicators decreased up to the 7th day of the experiment and increased up to the 28th day, not reaching the control level, except for the superoxide dismutase activity of the heart, which was normalized in females. At all terms of the post-traumatic period, the studied indicators in females were statistically significantly higher than in males. Under the influence of the correction, the superoxide dismutase and catalase activity of the heart became higher in females and males compared to rats without correction. At the same time, the degree of increase in the investigated parameters under the conditions of correction was significantly higher in males than in females.
Conclusions. Under the influence of cranioskeletal trauma in male rats, there is a higher exhaustion of the antioxidant defense enzyme link, which is detected on the basis of a greater decrease in the control of superoxide dismutase and catalase activity of the heart compared to female rats. The use of 2-ethyl-6-methyl-3-hydroxypyridine succinate for the purpose of correction in rats of different sexes helps to protect the disorders of the studied parameters, the degree of which is significantly higher in male rats.
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