CHARACTERISTICS OF INDICATORS OF OXIDATIVE STRESS OF MEN SUFFERED AS A RESULT OF COMBAT ACTIONS

Authors

  • M. Z. Vorobets DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • O. K. Onufrovych DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • Z. D. Vorobets DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • A. S. Besedina DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • O. V. Melnyk DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • R. V. Fafula DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • D. Z. Vorobets DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2023.i4.14374

Keywords:

male combat casualties, reduced glutathione, total glutathione, oxidized glutathione, malondialdehyde, total antioxidant activity

Abstract

Introduction. In a number of works, it was established that oxidative stress leads to a decrease in the fertilizing ability of spermatozoa, their damage and is one of the factors associated with male infertility. Normally, the balance between pro-oxidant and antioxidant processes is maintained. In a pathological condition, this balance is disturbed in the direction of uncontrolled generation of free radicals. The antioxidant defense system includes both enzymatic and non-enzymatic components that neutralize ROS and free radicals and protect against excessive exposure to oxidative stress.

 The aim of the study – to assess the state of lipid peroxidation and the non-enzymatic link of the glutathione antioxidant system of lymphocytes and peripheral blood plasma in men injured as a result of combat operations (bullet and shrapnel wounds).

 Research Methods. Research was conducted on lymphocytes and serum of peripheral blood of men injured as a result of hostilities, because lymphocytes are considered the "metabolic mirror" of the body and they quickly respond to all external and internal influences. The concentration of malondialdehyde, total antioxidant activity, concentrations of reduced, total and oxidized glutathione were determined.

 Results and Discussion. It was found that the concentration of reduced glutathione in the blood serum of men injured as a result of hostilities, the 1st age group (20–39 years old) decreased by 1.4 times, and for the second (40–53 years old) – by 1.6 times compared to control values. The concentration of total glutathione decreases for both affected age groups by 1.2 times. At the same time, no significant changes in the concentration of oxidized glutathione were detected. Determination of similar parameters of the non-enzymatic link of the glutathione antioxidant system in blood lymphocytes showed similar patterns as in blood serum. The calculation of the redox index (RI GSH) showed a decrease in the total capacity of the antioxidant system in the blood lymphocytes of men affected by hostilities by 1.5 times compared to the control group.

 Conclusion. In men who suffered as a result of combat (shrapnel and bullet wounds) and are undergoing inpatient treatment, both in lymphocytes and blood serum, lipid peroxidation processes are intensified, the concentration of reduced glutathione is reduced, and the ratio of restored glutathione to oxidized glutathione is reduced, which indicates a violation of pro-oxidant antioxidant balance in the direction of increasing pro-oxidant processes.

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Published

2024-01-09

How to Cite

Vorobets, M. Z., Onufrovych, O. K., Vorobets, Z. D., Besedina, A. S., Melnyk, O. V., Fafula, R. V., & Vorobets, D. Z. (2024). CHARACTERISTICS OF INDICATORS OF OXIDATIVE STRESS OF MEN SUFFERED AS A RESULT OF COMBAT ACTIONS. Medical and Clinical Chemistry, (4), 50–58. https://doi.org/10.11603/mcch.2410-681X.2023.i4.14374

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No. 4 (2023)

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ORIGINAL INVESTIGATIONS

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