CHARACTERISTICS OF INDICATORS OF OXIDATIVE STRESS OF MEN SUFFERED AS A RESULT OF COMBAT ACTIONS

Authors

  • M. Z. Vorobets DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • O. K. Onufrovych DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • Z. D. Vorobets DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • A. S. Besedina DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • O. V. Melnyk DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • R. V. Fafula DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • D. Z. Vorobets DANYLO HALITSKY LVIV NATIONAL MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2023.i4.14374

Keywords:

male combat casualties, reduced glutathione, total glutathione, oxidized glutathione, malondialdehyde, total antioxidant activity

Abstract

Introduction. In a number of works, it was established that oxidative stress leads to a decrease in the fertilizing ability of spermatozoa, their damage and is one of the factors associated with male infertility. Normally, the balance between pro-oxidant and antioxidant processes is maintained. In a pathological condition, this balance is disturbed in the direction of uncontrolled generation of free radicals. The antioxidant defense system includes both enzymatic and non-enzymatic components that neutralize ROS and free radicals and protect against excessive exposure to oxidative stress.

 The aim of the study – to assess the state of lipid peroxidation and the non-enzymatic link of the glutathione antioxidant system of lymphocytes and peripheral blood plasma in men injured as a result of combat operations (bullet and shrapnel wounds).

 Research Methods. Research was conducted on lymphocytes and serum of peripheral blood of men injured as a result of hostilities, because lymphocytes are considered the "metabolic mirror" of the body and they quickly respond to all external and internal influences. The concentration of malondialdehyde, total antioxidant activity, concentrations of reduced, total and oxidized glutathione were determined.

 Results and Discussion. It was found that the concentration of reduced glutathione in the blood serum of men injured as a result of hostilities, the 1st age group (20–39 years old) decreased by 1.4 times, and for the second (40–53 years old) – by 1.6 times compared to control values. The concentration of total glutathione decreases for both affected age groups by 1.2 times. At the same time, no significant changes in the concentration of oxidized glutathione were detected. Determination of similar parameters of the non-enzymatic link of the glutathione antioxidant system in blood lymphocytes showed similar patterns as in blood serum. The calculation of the redox index (RI GSH) showed a decrease in the total capacity of the antioxidant system in the blood lymphocytes of men affected by hostilities by 1.5 times compared to the control group.

 Conclusion. In men who suffered as a result of combat (shrapnel and bullet wounds) and are undergoing inpatient treatment, both in lymphocytes and blood serum, lipid peroxidation processes are intensified, the concentration of reduced glutathione is reduced, and the ratio of restored glutathione to oxidized glutathione is reduced, which indicates a violation of pro-oxidant antioxidant balance in the direction of increasing pro-oxidant processes.

References

Melnyk, O., Vorobets, M., Onufrovych, O., Be­sedina, A., Fafula, R., & Vorobets, Z. (2022). Relationship between indicators of oxidative stress and idiopathic male infertility. Experimental and clinical me­dicine, 91(2), 6-15. https://doi.org/10.35339/ekm.2022.91.2.mvo [in Ukrai­nian]. DOI: https://doi.org/10.35339/ekm.2022.91.2.mvo

Fafula, R.V., Onufrovych, O.K., Yefremova, U.P., Nakonechny, Y.A., & Vorobets, Z.D. (2017). Intensity of lipoperoxidation processes in spermatozoa of men with impaired fertility. Herald of problems of biology and medicine, (1), 199-204 [in Ukrainian].

Adeoye, O., Olawumi, J., Opeyemi, A., & Christiania, O. (2018). Review on the role of glutathione on oxidative stress and infertility. JBRA assisted reproduction, 22(1), 61-66. https://doi.org/10.5935/1518-0557.20180003 [in English]. DOI: https://doi.org/10.5935/1518-0557.20180003

Agarwal, A., Parekh, N., Panner Selvam, M.K., Henkel, R., Shah, R., Homa, S.T., Ramasamy, R., et al. (2019). Male Oxidative Stress Infertility (MOSI): Proposed Terminology and Clinical Practice Guidelines for Management of Idiopathic Male Infertility. The world journal of men’s health, 37(3), 296-312. https://doi.org/10.5534/wjmh.190055 [in English]. DOI: https://doi.org/10.5534/wjmh.190055

Aitken, R.J., Drevet, J.R., Moazamian, A., & Gharagozloo, P. (2022). Male Infertility and Oxidative Stress: A Focus on the Underlying Mechanisms. Antioxidants (Basel, Switzerland), 11(2), 306. https://doi.org/10.3390/antiox11020306. DOI: https://doi.org/10.3390/antiox11020306

Aitken, R.J. (2020). Impact of oxidative stress on male and female germ cells: implications for fertility. Reproduction (Cambridge, England), 159(4), R189–R201. https://doi.org/10.1530/REP-19-0452 [in English]. DOI: https://doi.org/10.1530/REP-19-0452

Ayaz, A., Agarwal, A., Sharma, R., Arafa, M., Elbardisi, H., & Cui, Z. (2015). Impact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men. Clinical proteomics, 12(1), 4. https://doi.org/10.1186/1559-0275-12-4 [in English]. DOI: https://doi.org/10.1186/1559-0275-12-4

Takalani, N.B., Monageng, E.M., Mohlala, K., Monsees, T.K., Henkel, R., & Opuwari, C.S. (2023). Role of oxidative stress in male infertility. Reproduction & fertility, 4(3), e230024. https://doi.org/10.1530/RAF-23-0024 [in English]. DOI: https://doi.org/10.1530/RAF-23-0024

Barati, E., Nikzad, H., & Karimian, M. (2020). Oxidative stress and male infertility: current knowledge of pathophysiology and role of antioxidant therapy in disease management. Cellular and molecular life sciences: CMLS, 77(1), 93-113. https://doi.org/10.1007/s00018-019-03253-8 [in English]. DOI: https://doi.org/10.1007/s00018-019-03253-8

Bisht, S., Faiq, M., Tolahunase, M., & Dada, R. (2017). Oxidative stress and male infertility. Nature reviews. Urology, 14(8), 470-485. https://doi.org/10.1038/nrurol.2017.69 [in English]. DOI: https://doi.org/10.1038/nrurol.2017.69

Vorobets, M.Z., Melnyk, O.V., Kovalenko, I.V., Fafula, R.V., Borzhievsky, A.T., & Vorobets, Z.D. (2021). Сondition of urogenital tract microbiotes and pro- and antioxidant system in male azoospermia. Regulatory Mechanisms in Biosystems, 12(4), 696-701. https://doi.org/10.15421/022196 [in English]. DOI: https://doi.org/10.15421/022196

Agarwal, A., Henkel, R., Sharma, R., Tadros, N.N., & Sabanegh, E. (2018). Determination of seminal oxidation-reduction potential (ORP) as an easy and cost-effective clinical marker of male infertility. Andrologia, 50(3), https://doi.org/10.1111/and.12914 [in English]. DOI: https://doi.org/10.1111/and.12914

Chaudhary, P., Janmeda, P., Docea, A.O., Yes­kaliyeva, B., Abdull Razis, A.F., Modu, B., Calina, D., & Sharifi-Rad, J. (2023). Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Frontiers in chemistry, 11, 1158198. https://doi.org/10.3389/fchem.2023.1158198 [in English]. DOI: https://doi.org/10.3389/fchem.2023.1158198

Fafula R, Melnyk O, Gromnatska N, Vorobets D., Fedorovych Z, A. Besedina, Z. Vorobets. (2023). Prooxidant-antioxidant balance in seminal and blood plasma of men with idiopathic infertility and infertile men in combination with rheumatoid arthritis. Studia Biologica. 17(2). P. 15-26. https://doi.org/10.30970/sbi.1702.719 [in ­English]. DOI: https://doi.org/10.30970/sbi.1702.719

Hosseinzadeh Colagar, A., Karimi, F., & Jorsa­raei, S.G. (2013). Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno- and oligoasheno-teratospermic men. Iranian Red Crescent medical journal, 15 (9), 780–785. https://doi.org/10.5812/ircmj.6409. DOI: https://doi.org/10.5812/ircmj.6409

Ko, E.Y., Sabanegh, E.S., Jr, & Agarwal, A. (2014). Male infertility testing: reactive oxygen species and antioxidant capacity. Fertility and sterility, 102(6), 1518-1527. https://doi.org/10.1016/j.fertnstert.2014. 10.020 [in English]. DOI: https://doi.org/10.1016/j.fertnstert.2014.10.020

Nabil H. Moemen L.A., Abuelela M. (2008). Studying the levels of malondialdehyde and antioxidant parameters in normal and abnormal human seminal plasma. Australian Journal of Basic and Applied Sciences, 2, 773-778. [in English].

Garrido, N., Meseguer, M., Simon, C., Pellicer, A., & Remohi, J. (2004). Pro-oxidative and anti-oxidative imbalance in human semen and its relation with male fertility. Asian journal of andrology, 6(1), 59-65.

Averill-Bates, D.A. (2023). The antioxidant glutathione. Vitamins and Hormones, 121, 109-141. https://doi.org/10.1016/bs.vh.2022.09.002. DOI: https://doi.org/10.1016/bs.vh.2022.09.002

Cacciatore, I., Cornacchia, C., Pinnen, F., Mollica A., & Di Stefano, A. (2010). Prodrug approach for increasing cellular glutathione levels. Molecules (Basel, Switzerland), 15 (3), 1242-1264. https://doi.org/10.3390/molecules15031242

Fafula, R.V., Onufrovych, O.K., Iefremova, U.P., Melnyk, O.V., Nakonechnyi, I.A., Vorobets, D.Z., & Vorobets, Z.D. (2017). Glutathione content in sperm cells of infertile men. Regulatory Mechanisms in Biosystems, 8 (2), 157-161. https://doi.org/10.15421/021725 DOI: https://doi.org/10.15421/021725

Forman, H.J., Zhang, H., & Rinna, A. (2009). Glutathione: overview of its protective roles, measurement, and biosynthesis. Molecular aspects of medicine, 30 (1-2), 1-12. https://doi.org/10.1016/j.mam.2008.08.006 DOI: https://doi.org/10.1016/j.mam.2008.08.006

Monostori, P., Wittmann, G., Karg, E., Túri, S. (2009). Determination of glutathione and glutathione disulfide in biological samples: an in-depth review. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci., 15, 877 (28), 3331-3346. https://doi.org/10.1016/j.jchromb.2009.06.016. Epub 2009 Jun 13. PMID: 19560987.

Hsieh, Y.Y., Chang, C.C., & Lin, C.S. (2006). Seminal malondialdehyde concentration but not glu­tathione peroxidase activity is negatively correlated with seminal concentration and motility. International Journal of Biological Sciences, 2(1), 23-29. https://doi.org/10.7150/ijbs.2.23. DOI: https://doi.org/10.7150/ijbs.2.23

Vorobets, M.Z., Fafula, R.V., Melnyk, O.V., Kovalenko, I.V., Borzhievsky, A.Ts., Vorobets, Z.D. (2022). Characteristics of oxidative stress and non-enzymatic link of the glutathione system in sperm plasma and spermatozoa in men with different fertilization potential. Experimental and Clinical Physiology and Biochemistry, 1/2 (94), 5-12. https://doi.org/10.25040/ecpb2022.01-02.005. DOI: https://doi.org/10.25040/ecpb2022.01-02.005

(2017). Public health care in war conditions. Kyiv: Nash format in 2 vol. [in Ukrainian].

Benedetti, S., Tagliamonte, M. C., Catalani, S., Primiterra, M., Canestrari, F., De Stefani, S., Palini, S., & Bulletti, C. (2012). Differences in blood and semen oxi­dative status in fertile and infertile men, and their rela­tionship with sperm quality. Reproductive biomedicine online, 25 (3), 300-306. https://doi.org/10.1016/j.rbmo. 2012.05.011. DOI: https://doi.org/10.1016/j.rbmo.2012.05.011

Henkel, R.R. (2011). Leukocytes and oxidative stress: dilemma for sperm function and male fertility. Asian journal of andrology, 13 (1), 43-52. https://doi.org/10.1038/aja.2010.76. DOI: https://doi.org/10.1038/aja.2010.76

Viloria, T., Meseguer, M., Martínez-Conejero, J.A., O’Connor, J. E., Remohí, J., Pellicer, A., & Garrido, N. (2010). Cigarette smoking affects specific sperm oxidative defenses but does not cause oxidative DNA damage in infertile men. Fertility and sterility, 94 (2), 631-637. https://doi.org/10.1016/j.fertnstert.2009.03.024 DOI: https://doi.org/10.1016/j.fertnstert.2009.03.024

Gavriliouk, D., & Aitken, R.J. (2015). Damage to Sperm DNA Mediated by Reactive Oxygen Species: Its Impact on Human Reproduction and the Health Trajectory of Offspring. Advances in experimental medicine and biology, 868, 23-47. https://doi.org/10.1007/978-3-319-18881-2_2 DOI: https://doi.org/10.1007/978-3-319-18881-2_2

Kalinina, E.V., Chernov, N.N., & Novichkova, M.D. (2014). Role of glutathione, glutathione transferase, and glutaredoxin in regulation of redox-dependent processes. Biochemistry. Biokhimiia, 79(13), 1562-1583. https://doi.org/10.1134/S0006297914130082 [in Ukrainian ]. DOI: https://doi.org/10.1134/S0006297914130082

Cacciatore, I., Cornacchia, C., Pinnen, F., Molli­ca, A., & Di Stefano, A. (2010). Prodrug approach for increasing cellular glutathione levels. Molecules (Basel, Switzerland), 15 (3), 1242-1264. https://doi.org/10.3390/molecules15031242 DOI: https://doi.org/10.3390/molecules15031242

Garrido, N., Meseguer, M., Alvarez, J., Simón, C., Pellicer, A., & Remohí, J. (2004). Relationship among standard semen parameters, glutathione peroxidase/glutathione reductase activity, and mRNA expression and reduced glutathione content in ejaculated spermatozoa from fertile and infertile men. Fertility and sterility, 82 Suppl 3, 1059-1066. https://doi.org/10.1016/j.fertnstert. 2004.04.033 DOI: https://doi.org/10.1016/j.fertnstert.2004.04.033

Lapovets, L., Lutsyk, B. (2004). Laboratory immunology. Кyiv: Aral [in Ukrainian].

Ayala, A., Muñoz, M.F., & Argüelles, S. (2014). Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative medicine and cellular longevity, 360438. https://doi.org/10.1155/2014/360438 DOI: https://doi.org/10.1155/2014/360438

Monostori, P., Wittmann, G., Karg, E., & Túri, S. (2009). Determination of glutathione and glutathione disulfide in biological samples: an in-depth review. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 877 (28), 3331-3346. https://doi.org/10.1016/j.jchromb.2009.06.016. DOI: https://doi.org/10.1016/j.jchromb.2009.06.016

Melnyk, O., Vorobets, M., Fafula, R., & Vorobets, Z. (2025). Association between sperm motility, oxidative stress and cytokines. Medicine Today and Tomorrow, 91 (2), 26-35. https://doi.org/10.35339/msz.2022.91.2.mvf [in Ukrainian]. DOI: https://doi.org/10.35339/msz.2022.91.2.mvf

Published

2024-01-09

How to Cite

Vorobets, M. Z., Onufrovych, O. K., Vorobets, Z. D., Besedina, A. S., Melnyk, O. V., Fafula, R. V., & Vorobets, D. Z. (2024). CHARACTERISTICS OF INDICATORS OF OXIDATIVE STRESS OF MEN SUFFERED AS A RESULT OF COMBAT ACTIONS. Medical and Clinical Chemistry, (4), 50–58. https://doi.org/10.11603/mcch.2410-681X.2023.i4.14374

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Section

ORIGINAL INVESTIGATIONS