CHANGES IN LIPID PEROXIDATION PROCESSES IN THE BLOOD OF STRESSED MALE RATS

Abstract

Introduction. The problem of stress is currently one of the main problems not only in Ukraine, but also in the whole world. True, not everyone is damaged by stress, some adapt to stress. Studying the impact of various types of stress, in particular, identifying the mechanisms of their impact on the body, is relevant.

The aim of the study – to evaluate the activity of lipid peroxidation processes in the blood of rats of various conditions that have undergone stress.

Research Methods. The study was performed on 48 white male Wistar rats aged 3.5–4 months. The animals were divided into 4 groups: 1 – control, 2 – stress 24 hours, 3 times (Stress 1), 3 – stress 24 hours, 4 times (Stress 2), 4 – stress 72 hours, 4 times (Stress 3). Stress was induced by gently fixing the animal with tissue on a board for 1 hour with its back down. Group 2 was stressed every 24 hours three times, group 3 was stressed every 24 hours four times, and group 4 was stressed every 72 hours four times. Superoxide dismutase and catalase activity were determined in blood serum (SOD, CAT), concentration of diene, triene conjugates (DC, TC), Schiff's bases (SB), TBA-active products (TBA-ap).

Results and Discussion. At Stress 1, due to control, DC, TC, OSH, TBA-ap were increased. The growth of SOD and CAT was noted. At Stress 2, due to the control, the content of DC increased and did not differ from animal group 2. TC in this group of animals became higher, than in control and were smaller, compared to group 2. The content of SB increased and was higher compared to animals of group 2. TBA-ap, respectively, with the control, increased, and were higher, respectively, with the 2nd group. Compared to the control, SOD did not change, and was smaller compared to group 2. CAT, in addition to the control, decreased, and was smaller, than in the animals of group 2. At Stress 3, compared to the control, the content of DC increased, and was higher, compared to group 2, and greater than animals of group 3. TC at Stress 3 were higher, respectively, in control, 2 and 3 groups of rats. The content of SB decreased, and was smaller, than in the animals of groups 2 and 3. TBA-ap, respectively, with the control, increased, and were higher, confirmed with 2 and 3 groups. Compared to the control, the SOD decreased sharply and was smaller compared to the Stress 1 and Stress 2 groups. The CAT, in accordance with the control, increased and was greater due to the animals of the 2 and 3 groups.

Conclusion. In the stress, which was repeated three times every 24 hours, the adaptation of the animals devepoled. In the stress, which was repeated four times every 24 hours, dysadaptation of animals is observed. In stress, repeated four times every 72 hours, exhaustion is observed in the animal.