DETECTION OF MIANSERIN IN URINE BY CHROMATOGRAPHIC METHODS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i3.14135Keywords:
antidepressants, mianserine, thin layer chromatography, high performance liquid chromatography, gas-liquid chromatography with mass spectrometryAbstract
Introduction. According to the literature, the greatest toxicological danger is formed by drugs that affect the central nervous system. According to toxicology services, about 800,000 fatal poisonings by psychotropic, hypnotic, sedative, antidepressant and other groups of drugs are registered annually in the world. In recent years, scientific interest in the study of antidepressants and narcotic substances in toxicological practice has grown significantly. However, every medicinal substance under certain conditions can become a poison, especially those that have a nature of long-term use, such as the tricyclic antidepressant mianserin.
The aim of the study – to develop methods for the identification of mianserine in biological fluids (urine) in the joint presence of thin layer chromatography (TLC), high performance liquid chromatography (HPLC), gas-liquid chromatography with mass spectrometry (Gl-MS).
Research Methods.The research used: chloroform solutions of mianserin and its metabolite desmethylmianserin M-7 isolated from the urine of poisoned animals; chromatographic systems: toluene-acetone-96 % ethyl alcohol-25 % ammonia solution (45: 45: 7,5: 2,5), chloroform-acetone (10:10), ethyl acetate-methanol-25 % ammonia solution (85: 10: 5), ethanol-methylene chloride- 25% ammonia solution (57.5: 40: 2.5); Marki and Dragendorf reagents; gas chromatograph Agilent 1200.
Results and Discussion. Identification of mianserine was performed by identification reactions, thin layer chromatography (TLC). The optimal chromatographic system is ethanol 96 %: methylene chloride: 25 % ammonia solution (57.5: 40: 2.5); developer – Mandeline reagent. The parameters of substance identification in these conditions are established: retention time, spectral ratios of substances, absorption spectra in UV spectra.
Conclusion. A method for detecting mianserine by TLC on Sorbfil PTSH plates has been developed. A method for the determination of mianserine using HPLC has been developed. A method for the identification of mianserine by gas-liquid chromatography with mass spectrometry (Gl-MS) has been developed. The optimal conditions for the isolation of mianserine in the joint presence of model mixtures of bioliquid (urine).
References
Schaper, A., Farber, E. (2012). Suicide with mianserine Toxicol. Clin. Toxicol., 40, 343-345.
Koski, A., Vuori, E., Ojanpera, I. (2015). Relation of postmortem blood alcohol and drug concentrations in fatal poisoning involving propoxyphene, mianserine and promazine Hum. Exp. Toxicol., 119 (6), 344-348.
Chin, B., Norio, N. (2020). Effects of mianserin and of its metabolites on plasma consentrations Therapeutic Dfug Monitoring, 21, 1661-1670.
Laimer, M., Kramer-Reinstadler, К., Rauchenzauner, М., & Lechner-Schoner Т. (2016). Effect of mianserin theatment on body composition and metabolism J. Clin Psychiatry, 67 (3), 421-424. DOI: https://doi.org/10.4088/JCP.v67n0313
Kelvin, A.S., & Hakansson, S.M. (2017) Somporative acute toxicity of mianserini and other antidepressants Agta Psychiatrica Scandinavica, 80 (23), 31-33. DOI: https://doi.org/10.1111/j.1600-0447.1989.tb07165.x
Kirchherr, H., Kuhn-Velten, W. (2016) Quantitative determination of forty-eight antidepressants and antipsychotiss in human serum by HPLC tandem mass spectrometry: a multilevel, single – sample approach J. Chromatogr B. Analyt. Teghnol., 20 (7), 100-113. DOI: https://doi.org/10.1016/j.jchromb.2006.05.031
Will, S., & Neels, H. (2017). Comparison of electron and shemical ionization modes by validation of a quantitative gas chromatographic – mass spectrometris assay of new generation antidepressants and their active metabolites in plasma J. Chromatigr A,1176 (1) , 236-242. DOI: https://doi.org/10.1016/j.chroma.2007.10.096
Novakova, E. (2014). Detection of new antidepressive agents using thin – layer chromatography. Soud Lek., 1, 2-6.
Goncharuk, N.V., & Halkevych, I.I. ( 2007). Application of the chromatography method in a thin layer of sorbent for the identification of tianeptine. Pharmaceutical Journal, 3, 37-38 [in Ukrainian].
Horlachuk, N.V., Mosula, L.M., Zarivna, N.O. (2018). Chromato-mass spectrometric analysis of tianeptine in urine. Medical and Clinical Chemistry, 4, 146-152 [ in Ukrainian]. DOI: https://doi.org/10.11603/mcch.2410-681X.2018.v0.i4.9828
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
