LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN WHITE RATS CONSUMING DRINKING WATER WITH DIFFERENT CONTENTS OF SODIUM MONOPHOSPHATE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i1.13583Keywords:
lipid peroxidation, antioxidant protection, phosphates, drinking waterAbstract
Introduction. The problem of anthropogenic pollution of surface water bodies with phosphates is one of the causes of eutrophication of water bodies, which leads to the accumulation of biotoxins, the deterioration of water quality, and negatively affects the physiological and biochemical processes of hydrobionts and harms human health. The starting mechanism of pathological processes in the body are membrane-damaging processes, among which lipid peroxidation (LPO) occupies a significant place.
The aim of the study – to establish the peculiarities of the course of LPO processes and the state of antioxidant protection (AOP) in the liver homogenate of white rats when drinking water with different concentrations of phosphates is used for 30 days.
Research Methods. Experiments were conducted on 36 white purebred male rats weighing 180–200 g, divided into 6 groups, which consumed water with the addition of sodium monophosphate in doses of 0.01; 0.1; 1.0; 10.0; 100.0 mg/dm3 in terms of elemental phosphorus. The content of TBA active products and diene conjugates (DK) was determined in the liver homogenate, and the state of AOP was assessed by the activity of superoxide dismutase (SOD) and catalase.
Results and Discussion. The obtained data indicate that concentrations of sodium monophosphate in drinking water at the level of 0.01, 0.1 and 1.0 mg/dm3 did not have a pronounced effect on the processes of POL in the body of rats. However, phosphates at the level of 10.0 and 100.0 mg/dm3 cause an increase in the content of TBK-AP and DC in the 4th group – by 90 and 73 % and in the 5th – by 92 % and 75 % (p<0, 05) respectively. It was also established that the activity of SOD decreased statistically significantly only in the 5th experimental group – by 45 % (p<0.05) and the activity of catalase increased statistically significantly in the 5th and 4th groups – by 56.5 % (p<0.05) and 34.2 % (p<0.1), respectively.
Conclusions. Drinking water with phosphates concentrations of 10.0 and 100.0 mg/dm3 has a negative effect on the state of cell membranes of hepatocytes due to the activation of POL processes. The detected changes in the body of experimental rats can be a specific pathogenic link in the formation of pathological changes in the body under simulated conditions, and the disruption of the AOP as a result of such an effect contributes to the strengthening of the destruction of membranes and the violation of their structural and functional properties.
References
Prokopov, V.O. (2016). Drinking water of Ukraine: medical-ecological and sanitary-hygienic aspects. Kyiv: Medicine [in Ukrainian].
National report on the quality of drinking water and the state of drinking water supply in Ukraine in 2020. Kyiv, 2021 [in Ukrainian].
Prokopchuk, O.I., & Grubinko, V.V. (2013). Phosphates in aquatic ecosystems. Scientific Issue Ternopil Volodymyr Hnatiuk National Pedagogical University. Series: Biology, 3(56), 78-85 [in Ukrainian].
Horova, O.O., & Samokhvalova, A.I. (2018). The problem of pollution of natural ecosystems with phosphates and ways to solve it. Abstracts of Papers of the 10th International Conference and the International EAS conference “Human safety in modern conditions” Kharkiv, December 6-7, 2018. (pp.17-19). Kharkiv: NTU “KhPI” [in Ukrainian].
Razzaque, M.S. (2011). Phosphate toxicity: new insights into an old problem. Clinical Science, 120 (3), 91-97. DOI.org/10.1042/CS20100377 DOI: https://doi.org/10.1042/CS20100377
Nieder, R., Benbi, D.K., & Reichl, F.X. (2018) Water-soluble forms of nitrogen and phosphorus and their impacts on environment and human health. In Soil Components and Human Health. (pp. 223–255). Springer, Dordrecht [in English]. DOI: https://doi.org/10.1007/978-94-024-1222-2_5
Simonova, N.A., & Mehed, O.B. (2018). Changes in the content of diene conjugates in the tissues of scaly carp under the toxic effects of synthetic detergent and phosphates. Proceedings of the of the 2-nd international scientific and methodical conference. Problems of ecology and evolution of ecosystems in the conditions of a transformed environment. Chernihiv, October 11-12. 2018. (pp. 121-123). Chernihiv: Desna Poligraf [in Ukrainian].
State sanitary norms and rules “Hygienic requirements for drinking water intended for human consumption” (DSanPiN 2.2.4-171-10) for 2010. [Electronic resource]. Access mode: http://zakon1.rada. gov.ua/cgi-bin/laws [in Ukrainian].
Govorukha, O.Yu., & Shnaiderman, O.Yu. (2016). The importance of the interaction of lipid peroxidation and antioxidant systems in the development of pathological processes. Experimental and Clinical Medicine, 4 (73), 10-14 [in Ukrainian].
Decker, E.A. (2002). Antioxidant mechanisms. In Food lipids: chemistry, nutrition, and biotechnology (Ed. 2, pp. 517-542). DOI: https://doi.org/10.1201/9780203908815.ch16
Stalnaya, I.D., & Harishvili, T.G. (1977). Method for determining malondialdehyde using thiobarbituric acid. In Modern Methods in Biochemistry (pp. 66-68). Moscow: Medicine [in Russian].
Stalnaya, I.D. (1977). Method for determining diene conjugation of unsaturated higher fatty acids. In Modern Methods in Biochemistry. Moscow: Meditsina [in Russian].
Chevary, S., Chaba, I., & Senei, I. (1988). The role of superoxide dismutase in the oxidative processes of cells and methods of its determination in biological material. Laboratory Work, 11, 678-681 [in Russian].
Korolyuk, M.A. (1988). The method of determining the activity of catalase Laboratory Work, 1, 6-19 [in Russian].
Lapach, S.N., Chubenko, A.V., & Babich, P.N. (2000). Statistical methods in biomedical research using Excel. Kyiv: Morion [in Russian].
Nagornaya, N.V., & Chetverik, N.A. (2010). Oxidative stress: influence on the human body, evaluation methods Health of the Child, 2 (23). [Electronic resource]. Access mode: http://pediatric.mif-ua.com/archive/issue-12604/article-12762[in Russian].
He, L., He, T., Farrar, S., Ji, L., Liu, T., & Ma, X. (2017) Antioxidants Maintain Cellular Redox Homeostasis by Elimination of Reactive Oxygen Species. Cell Physiol. Biochem., 44, 532-553. DOI: 10.1159/000485089. DOI: https://doi.org/10.1159/000485089
Zheng, H., Wu, J., Jin, Z., & Yan, L-J. (2017). Potential biochemical mechanisms of lung injury in diabetes. Aging and Disease, 1 (8), 7-16. DOI: https://doi.org/10.14336/AD.2016.0627
Grune, T., & Berger, M.M. (2007). Markers of oxidative stress in ICU clinical settings: present and future. Current Opinion in Clinical Nutrition & Metabolic Care, 10(6), 712-717. DOI: https://doi.org/10.1097/MCO.0b013e3282f0c97c
Diorditsa, Ya. (2019). Antioxidant system of the liver of rats under conditions of acute hepatitis during correction with antioxidant complexes. Visnyk of Lviv University. Biological Series. 81, 12-20 [in Ukrainian]. DOI: https://doi.org/10.30970/vlubs.2019.81.02
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
