FEATURES OF LIPOPEROXIDATION PROCESSES IN THE PROSTATE AT CHRONIC ALCOHOL INTOXICATION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i4.13566Keywords:
prostate, lipoperoxidation, chronic alcohol intoxicationAbstract
Introduction. Chronic alcohol intoxication leads to damage to almost all organs and systems, the degree of structural and functional disorders of which in this pathology is different and depends on the duration and severity of intoxication.
The aim of the study – to learn the features of lipoperoxidation processes in the prostate gland during chronic alcohol intoxication.
Research Methods. Biochemical and morphometric methods investigated the structures of the prostate of 60 laboratory adult white male rats, which were divided into 2 groups. Group 1 included 30 intact experimental animals, group 2 included 30 rats that were at chronic alcohol intoxication. A month after the start of the experiment, the experimental animals were euthanized by bloodletting under thiopental anesthesia. The content of diene conjugates, active products of thiobarbituric acid, was determined in prostate homogenates. Correlation analysis was carried out between biochemical and studied histostereometric parameters with determination of correlation coefficient (r). Quantitative indicators were processed statistically.
Results and Discussion. It was established that in conditions of chronic ethanol intoxication, the processes of lipoperoxidation in the tissues of the prostate significantly increase. The level of diene conjugates increased by 2.3 times (p<0.001), the concentration of active products of thiobarbituric acid by 2.4 times (p<0.001). Stromal-parenchymatous relation in chronic ethanol intoxication in the studied organ increased by 1.57 times, nuclear-cytoplasmic ratios in endotheliocytes – by 7.6 %, epitheliocytes of glands – by 9.6 % (р<0.001), the relative volume of damaged endotheliocytes – 11.66 times, gland epitheliocytes – 19.7 times (p<0.001). Strong positive correlations between biochemical and morphometric indicators were revealed.
Conclusions. Chronic alcohol intoxication leads to a marked increase in peroxide oxidation of lipids in prostate tissues and damage to the endotheliocytes of arteries and epithelial cells of glands, infiltrative and sclerotic processes. The degree of lipoperoxydation disorders in simulated pathology correlates with the severity of changes in structural homeostasis, the relative volumes of damaged endotheliocytes, glandular epitheliocytes, and stromal-parenchymal relations in the organ under study.
References
Budnik, A.F., Bogatyreva, O.E., & Musukaeva, A.B. (2016). Morphological characteristics of the human prostate in chronic alcohol intoxication. International Research Journal, 3 (45), 50-52 [in Russian].
Hrytsulyak, B.V., Hrytsulyak, V.B., Pastukh, M.B., & Dolinko, N.P. (2014). Histo-and ultrastructural changes in the testis of rats with chronic alcohol intoxication. World of Medicine and Biology, 2 (44), 114-117 [in Ukrainian].
Kechagias, S., Dernroth, D., & Blomgren A. (2015). Phosphatidylethanol compared with other blood tests as a biomarker of moderate alcohol consumption in healthy volunteers: A prospective randomized study. Alcohol and Alcoholism, 50 (4), 399-406. DOI: 10.1093/alcalc/agv038.
Smolina, S.P., Petrova, M.M., Sharobaro, V.I., & Nikolaev, S.V. (2016). Oxidative distress in the pathogenesis of alcoholic liver disease and ways of its correction. General Resuscitation, 12 (1), 35-42 [in Russian].
Kudo, R., Yuui, K., Kasuda, S., & Hatake, K. (2015). Effect of alcohol on vascular function. Nihon Arukoru Yakubutsu Igakkai Zasshi, 50 (3), 123-134.
Ustenko, R.L., Sherstyuk, О.А., & Pilyugin, A.V. (2015). Stereomorphological features of the structure of the human prostate and the complexity of its nomenclature. Galician Medical Bulletin, 22(3), part 2, 111-114 [in Russian].
Shepitko, V.I., Vilkhova, O.V., Soguyko, Y.R., & Soguyko, R.R. (2015). Structural organization of the prostate gland. Bulletin of Problems of Biology and Medicine, 2 (125), 59-62 [in Ukrainian].
Hrytsulyak, B.V., Dolinko, N.P., Popadynets, O.G., & Pastukh, M.B. (2016). The nature of morphofunctional changes in the prostate caused by ethanol intoxication. Bulletin of Problems Biology and Medicine, 2 (129), 70-73 [in Ukrainian].
Denefil, O.V., Medynskyi, M.I., Saliy, Z.V., Kulitska, M.I., Andriichuk, I.Ya., Usynskyi, R.S. (2022). The role of the processes of peroxidic oxidation of lipids in the heart of rats of different sexes in the dynamics of the development of craniocerebral trauma. Medical and Clinical Chemistry, 24 (3), 27-32 [in Ukrainian].
Hnatjuk, M.S., & Tatarchuk, L.V. (2018). Morphometric features of remodeling of the ileum membrane in postresection portal hypertension. Bulletin of Problems Biology and Medicine, 2 (147), 258-261 [in Ukrainian].
Kovalev, G.A., & Petrenko, A.Yu. (2004). Experimental model of alcoholic liver damage in female rats. Visnyk Khark. National University, 617, 15-18 [in Ukrainian].
Bagriy, M.M., Dibrova, V.A., Popadynets, O.G., & Grishchuk, I.M. (2016). Methods of morphological research. Vinnytsia: Nova knyha [in Ukrainian].
Grzhibovsky, A.I., Ivanov, O.V., & Gorbatova, M.A. (2016). Comparison of quantitative data of two pair samples using the software Statistica and SPSS: parametric and nonparametric criteria. Science and Health, 3, 5-25. DOI:1034689/SH/2026.18.3001 [in Ukrainian].
Zaporozhyan, V.M., & Aryaev, M.L. (2013). Bioethics and biosafety. Kyiv: Zdorovia [in Ukrainian].
Malyarskay, N.V., & Kalinichenko, M.A. (2017). Endothelial dysfunction as a universal predictor of the development of cardiovascular pathology and the possibility of its correction in the practice of a family doctor. Drags of Ukraine, 1 (207), 38-41 [in Ukrainian].
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