DEVELOPMENT OF OXIDATIVE STRESS IN THE HEART OF DIFFERENT SEXES RATS IN THE DYNAMICS OF THE DEVELOPMENT OF SKELETAL TRАUMA

Abstract

Introduction. Recently, the number of people with field injuries has increased significantly, but skeletal trauma remains relevant. Men with polytraumas are characterized by a higher risk in all clinical and nosological groups compared to women. The leading link in the development of pathology remains oxidative stress, which affects the development of cardiovascular pathology.

The aim of the study was to evaluate the development of oxidative stress in the heart homogenate of rats of different sexes in different periods of development of skeletal trauma.

Research methods. Experiments were performed on 80 outbred rats of different sexes weighing 180-220 grams. Animals were divided into 2 groups - control, skeletal trauma. Trauma was performed in animals under sodium thiopental anesthesia by a single dosed blow to the right thigh. Slaughter of animals was carried out in control, 1, 7, 14 and 28 days after injury. A heart sample was taken, in the homogenate of which diene conjugates (DC), TBA-active products (TBA-ap), superoxide dismutase (SOD) and catalase activity (Cat) were determined. A morphological study of the myocardium was carried out in Heidenhain-stained preparations.

Results and discussion. In control rats-males, compared to females, lipid peroxide oxidation products predominated, but superoxide dismutase and catalase activities were lower. During the experiment, an increase in DC and TBA-ap indicators was noted to a greater extent in males, while SOD and Cat activity remained higher in females.

Conclusion. The development of oxidative stress in the heart of rats with skeletal trauma depends on the initial activity of the antioxidant system and gender. More pronounced damaging changes were noted in males. Greater activity of antioxidants prevents significant damage to the myocardium.