ACTIVITY OF LIPID PEROXIDATION PROCESSES IN THE HEART OF MALE RATS AFTER CASTRATION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i2.13209Keywords:
lipid peroxidation, antioxidant system, heart, castrationAbstract
Introduction. Pathology of the cardiovascular system takes a leading place in the structure of morbidity and mortality. One of the reasons for its development is sex hormones.
The aim of the study – to evaluate the development of oxidative stress in the heart homogenate of male rats after castration and replacement therapy.
Research Methods. Experiments were performed on Wistar rats of 160–180 grams. The animals were divided into 5 groups – control, castration for 1 month, castration for 2 months, castration + replacement therapy for 1 month, castration + replacement therapy for 2 months. Hormone replacement therapy in castrated animals was reproduced with testosterone propionate ("Farmak") 1 mg/kg subcutaneously once a day throughout the experiment. The content of diene conjugates (DC), TBK-active products (TBK-ap), superoxide dismutase activity (SOD), catalase activity was determined in the heart homogenate.
Results and Discussion. In the group of animals that were castrated a month ago, the content of DC and TBC-active products, compared to the control, decreased, and in rats that were castrated two months ago, they increased. Replacement therapy led to modulatory effects of the content of DC and TBA-active products: one-month therapy led to an increase, and two-month therapy led to a decrease in indicators. DC values during replacement therapy were not significantly different from controls, but were higher after a 2-month course of replacement therapy. The values of the index of TBC-active products during replacement therapy did not depend on the duration of the course and did not differ reliably from the control. The enzyme link of the antioxidant system increased 1 month after castration, and decreased 2 months later. Replacement therapy showed a modulating effect, causing the normalization of SOD and catalase activity.
Conclusion. Castration of male rats after 1 month causes an increase in SOD and catalase activity, a decrease in lipid peroxidation processes compared to the control. Castration of male rats after 2 months causes a decrease in SOD and catalase activity, an increase in lipid peroxidation processes compared to the control. Carrying out replacement therapy with sex hormones in castrated animals brings the studied biochemical indicators closer to the level of the control group of rats.
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