CORRECTIVE EFFECT OF THE PEPTIDE ON ANTIOXIDANTION SYSTEM IN MALE RATS WITH ROUNDUP AND HEAVY METALS POISONING
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i2.13208Keywords:
lead acetate, copper sulfate, glyphosate, antioxidant system, glutathione systems enzymesAbstract
Introduction. Lead, copper and other heavy metals are one of the most common environmental pollutant and potential dangerous agent to human health. Most of the lead entering the systemic circulation invades into the bone marrow, bone, spleen, liver and kidney. Prolonged exposure of this toxicant causes oxidative stress, damage of DNA and considered a risk factor for kidney, liver and many disorders. Copper and its salts have a similar effect.
The aim of the study – to investigate the toxic effects of lead acetate, copper sulfate and glyphosate (roundup) on the indicators of the antioxidant system in different age groups rats and try to diminish this toxicity by peptide (cysteyl-histidyl-tyrosyl-histidyl-isoleucine) and unithiol.
Research Methods. The experiment was on lab nonlinear white rats – males three age periods: puberty, mature and old aging animals. The rats receiving the lead acetate, copper sulfate, glyphosate (in herbicide Roundup) in combined and peptide as correction agent. Subhronic lesions in rats modelled by intragastric administration of water solution of Lead Acetate at a dose of 11 mg/kg, Copper Sulfate at a dose of 13 mg/kg, Glyphosate at a dose of 250 mg/kg. Dechlorinated drinking tap water to intact animals added. The correction effect was research by the during 10 days intragastric administration of water solution of peptide cisteil-histidil-tyrosil-histidil-isoleucine and unithiol at 21st day of the experiment after 6 hours after toxins. The activity of glutathioneperoxidase, glutathione reductase, catalase, superoxide dismutase and the content of SH-Groups was determined in blood serum and liver homogenates of rats.
Results and Discussion. In this study, the effects of lead acetate, copper sulfate and roundup on the indicators of the antioxidant system in different age groups rats in the absence and presence of peptide and unithiol were analyzed. The results of study evoked a significant decrease in the blood serum and in the liver homogenate levels GSH-groups with lead acetate, copper sulfate and glyphosate (roundup) poisoning rats. Lead acetate; copper sulfate; glyphosate intoxication significantly affected on the activity glutathioneperoxidase, glutathione reductase, catalase, superoxide dismutase and the content of SH-Groups of treated animals. The main effects in the case of lead acetate; copper sulfate; glyphosate intoxication were decreased values of SH-Groups and activity antioxidant system enzymes compared with the control group. The treatment with peptide (cisteil-histidil-tyrosil-histidil-isoleucine) and unithiol reduced the toxic effects of these metals and raundap in all cases. The ability of peptide to reduce heavy metals and glyphosate (roundup) toxicity may relate to its antioxidant actions or enhancing, the chelation of metal.
Conclusion. The present study proved the cysteyl-histidyl-tyrosyl-histidyl-isoleucine showed effective anti-oxidative action in poisoning lead acetate, copper sulfate and glyphosate (roundup) rats. Therefore, the peptide as corrective agent can recommend using in case with heavy metal poisoning.
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