CHANGES OF INDEXES OF CARBOHYDRATЕ ERYTHROCYTES OF RATS UNDER CONSUMPTION OF ENERGY DRINK

Abstract

introduction. In recent years, there has been an increase in energy consumption among adolescents around the world and in Ukraine. Excessive consumption of energy drinks can lead to excessive sugar consumption, which in turn causes long-term health problems.

The aim of the study – to investigate the change in carbohydrate metabolism of rat erythrocytes under conditions of energy consumption.

Materials and Methods. The study was conducted using male Wistar rats, which were kept in the vivarium under appropriate lighting conditions, temperature, humidity and standard diet. All animals had free access to feed (based on daily requirements) and water (based on 20 ml of water per rat per day). The amount of standard feed consumed for laboratory animals was determined by its residue in the feeder. Control over the growth and development of animals was performed by weighing them at the beginning and at the end of the experiments. The experimental animals were divided into five groups: group 1 – received drinking water (control group); group 2 – received an energy drink for a month and the collection of material was carried out on the 1th day at the end of the experiment; group 3 – received an energy drink for a month and the collection of material was carried out on the 10th day at the end of the experiment; group 4 – received an energy drink for a month and the collection of material was carried out on the 20th day at the end of the experiment; group 5 – received an energy drink for a month and the collection of material was carried out on the 30th day at the end of the experiment. Glucose concentration was determined by glucose oxidase method; pyruvic acid – by the number of derivatives of 2,4 dinitrophenylhydrazone; lactic acid by reaction with paraoxydiphenyl. The activity of the enzyme lactate dehydrogenase was determined by enzymatic method. Studies of the level of ATP in the hemolysate of erythrocytes were performed according to the method of Oleynikova.

Results and Discussion. It was found that against the background of energy drink there was an increase in the level of metabolites of carbohydrate metabolism in erythrocytes of experimental groups of animals: glucose, pyruvate, lactate, ATP and lactate dehydrogenase activity.

Conclusions. The results indicate a violation of carbohydrate metabolism in the erythrocytes of animals that consumed energy, which can lead to disruption of both homeostasis of red blood cells and the body as a whole.