THE EFFECT OF ZINC SULFATE, SODIUM THIOSULFATE, LIPOIC ACID, AND TAURINE ON HYDROGEN SULFIDE METABOLISM IN KIDNEYS OF RATS WITH DIET-INDUCED OBESITY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i1.13036Keywords:
hydrogen sulfide, metabolism, obesity, chronic kidney disease, high-calorie high-fat diet, ratsAbstract
Introduction. The problem of prevention and correction of nephropathy in obesity is becoming increasingly important. The kidneys synthesize a multifunctional regulator of hydrogen sulfide (H2S), the metabolism of which in obesity can accelerate the formation of chronic kidney disease. The search for metabolic correctors that could normalize H2S metabolism in the kidneys in obesity and would not show a lipogenic effect remains relevant. This effect can be seen in cofactors and co-substrates of thiosulfate-dependent H2S metabolism.
The aim of the study – to establish the effect of zinc sulfate, sodium thiosulfate, lipoic acid, and taurine on hydrogen sulfide metabolism in kidneys of rats with diet-induced obesity (DIO).
Research Methods. Experiments were performed on 60 white laboratory male rats in accordance with the principles of bioethics (Strasbourg, 1986; Kyiv, 2001). DIO was induced in 50 rats by dieting on a high-calorie high-fat diet (energy value 4.33 kcal/g) for 10 weeks. The control group (10 rats) received a standard diet (energy value 2.71 kcal/g). Correctors of H2S metabolism (zinc sulfate, sodium thiosulfate, lipoic acid, taurine) were administered during the last 2 weeks. The activity of the enzymes of H2S metabolism was determined in kidney homogenates. Statistical processing was performed in the package IBM Statistics SPSS 26, differences were assessed in the Kraskel – Wallis test at a significance level of p<0.05.
Results. The following H2S metabolism disorders in the kidneys were detected in DIO rats: decreased activity of PLP-dependent transsulfuration enzymes (cystathionine-γ-lyase, cystathionine-β-synthase, cysteine aminotransferase), decreased activity of thiosulfate-dependent pathways of H2S metabolism (thiosulfate (thiol) sulfurtransferase, thioredoxin reductase, sulfite oxidase) and low H2S level, which correlated with an increase in body mass index and index Lee. Zinc sulfate, lipoic acid, sodium thiosulfate and taurine increased the level of H2S and the activity of H2S-synthesizing enzymes of transsulfuration and thiosulfate-dependent pathways in the kidneys (1.4–1.5 times, p <0.01), without causing lipogenic effect in rats with DIO.
Conclusions. Zinc sulfate, lipoic acid, sodium thiosulfate effectively reduce H2S metabolism disorders in the kidneys which were induced by high-calorie high-fat diet and inhibit the development of obesity in rats.
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