CHEMICAL COMPOSITION OF THE EQUIVALENT OF THE CORNEA STROMA OBTAINED BY THE DECELULARIZATION METHOD
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i4.12733Keywords:
pig cornea, decellularization, macro- and microelements, atomic absorption method of analysis, keratoplastyAbstract
Introduction. As a result of increasing military, traffic and domestic injuries, the problem of shortage of donor material is particularly relevant. The huge shortage of donor material for keratoplasty forces us to look for additional sources of transplant material, methods of making and using xenogeneic grafts. One such material is the pig's cornea, which is similar in structure and biomechanical parameters to the human cornea.
The aim of the study – to research the chemical composition of the equivalent of the corneal stroma, removed from the eyes of pigs and obtained by the method of decelularization in order to further its use as a material for keratoplasty.
Research Methods. The corneal membrane obtained from the removed eyes of pigs is placed in a medium for tissue culture, followed by its decellularization. Treated with 0.5 % solution of sodium dodecyl sulfate with constant shaking; treated with ultrasound (three times), incubate in the presence of an enzyme solution of papain; washed with buffer solution (pH 6.5), centrifuged. Place in a solution of polyvinylpyrrolidone for storage. Photocolorimetric and atomic absorption methods of analysis were used to determine the elemental composition of biological samples.
Results and Discussion. The chemical composition of the equivalent of the corneal stroma obtained by the method of decelularization was studied. According to the results of atomic absorption analysis in the decellularized cornea of pig, 14 macro- and microelements were identified. Among macroelements, the largest amount is sodium – 8323 mg/kg of air-dry sample, potassium – 1163 mg/kg, magnesium – 618 mg/kg and calcium – 224 mg/kg. The mass fraction of microelements in the study object is in the following sequence: iron – 755 mg/kg, cobalt – 296 mg/kg, nickel – 103 mg/kg, titanium – 13.2 mg/kg, zinc – 7 .9 mg/kg, manganese – 4.5 mg/kg, silicon – 6.2 mg/kg, copper – 5.7 mg/kg, lanthanum – 0.4 mg/kg. The smallest amount is argentum – 0.03 mg/kg.
Conclusion. Promising for further research is a comprehensive study and improvement of the method used to deceleralize the cornea of animals in order to obtain bioengineered samples for their subsequent keratoplasty, which solves the problem of transplantation – obtaining donor material.
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