DYNAMICS OF THE ACTIVITY OF LIPID PEROXIDATION PROCESSES IN LATE PERIOD OF CRANIOSKELETAL INJURY IN CASE OF CHRONIC HEPATITIS AND THE EFFECTIVENESS OF CORRECTION WITH ARMADINE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i4.12728Keywords:
cranioskeletal injury, traumatic disease, late period, lipid peroxidation, armadineAbstract
Introduction. Currently, injuries are considered as relevant medical and social problem of the nowadays. Recently, the probability of developing traumatic disease has increased significantly based on the background of concomitant diffuse liver disease, the frequency of which is constantly increasing. One of the objects of the body's systemic response to injury is the liver, where lipid peroxidation is significantly enhanced due to traumatic disease.
The aim of the study – to investigate the dynamics of lipid peroxidation processes in late period of cranioskeletal trauma (CST) in case of chronic hepatitis and the effectiveness of correction with armadine.
Research Methods. The experiments were performed on 136 nonlinear white male rats weighting 200–220 g. In the first experimental group (EG-1) after the modelling of chronic hepatitis (Siegers C. P. et al., 1982), cranioskeletal trauma (CST) was simulated. In the second experimental group (EG-2) only CST was modeled. In the third study group (EG-3), chronic hepatitis was induced, CST was simulated and armadine at a dose of 20 mg/kg was administered intraperitoneally every day for correction starting from 15 days after CST. Animals of the experimental groups were removed from the experiment 14, 21, 28 and 35 days after injury. In the control and experimental groups, the content of reagents for thiobarbituric acid (TBA-active products of LPO) was determined in the liver.
Results and Discussion. Modelling of CST in the period of late manifestations of traumatic disease is accompanied by activation of lipid peroxidation processes in the liver. The content of TBA-active products of LPO in the liver throughout the experiment was significantly higher than in the control group. Under the conditions of application of CST with the concomitant chronic hepatitis, the content of TBA-active products of LPO in the liver increased and significantly exceeded the group of injured animals without concomitant chronic hepatitis. The use of armadine helped to reduce the intensity of lipid peroxidation in the liver under the conditions of CST on the background of chronic hepatitis. Compared with animals without correction, the result was statistically significant after 21 and 35 days.
Conclusions. Modeling of CST on the background of chronic hepatitis in the period of late manifestations of traumatic disease causes a prolonged increase in the activity of lipid peroxidation in the liver, which up to 35 days of the experiment does not reach the level of control. The use of armadine compared with animals without correction causes a decrease in the content of TBA-active products of the liver starting from the second day of the experiment.
References
Sarychev, L.P., Sarychev, Y.V., Pustovoyt, H.L., Sukhomlin, S.A., & Suprunenko, S.M. (2018). Management of the patients with blunt renal trauma: 20 years of clinical experience. Wiad. Lek., 71 (3 pt. 2), 719-722.
Huriev, S.O., Fil, A.Yu., & Tanasienko, O.M. (2015). Analysis of immediate causes of death in victims with polytrauma and skeletal damage. Trauma, 16 (4), 7-10 [in Ukrainian].
Hudyma, A.A., & Zayets, T.A. (2014). Features of absorption-excretory and glycogen-synthesizing functions of the liver in the early period of cranioskeletal trauma and their correction by cell therapy. Bull. Sci. Res., 2, 97-99.
Kozak, D.V. (2013). Features of the functional state of the liver in the dynamics of the early post-traumatic period of trauma. Biomed. Biosoc. Anthropology, 20, 97–99 [in Ukrainian].
Shcherbina, M.B. (2012). Innovations in the treatment of chronic drug and alcoholic hepatitis. Gastroenterology, 429, 48–50.
Blyzniuk, R.V. (2015). Dynamics of lipid peroxidation and antioxidant protection indicators of liver tissue in the early period after the application of multiple injuries in animals with chronic hepatitis. Achievements of Clinical and Experimental Medicine, 2-3, 32-36. DOI: 10.11603/ 1811-2471.2015.v23.i2-3.5233.
Hudyma, A.A., Kashchak, T.V., & Shepitko, K.V. (2019). Antioxidant-prooxidant and cytokine balance in the late period of combined trauma in the experiment. World of Medicine and Biology, 1 (67), 42-47. DOI: 10.26724/2079-8334-2019-1-67-42 [in Ukrainian].
Deryugina, A.V., Shumilova, A.V., Filippenko, E.S., Galkina, Y.V., Simutis, I.S., & Boyarinov, G.A. (2017). Functional and biochemical parameters of erythrocytes during mexicor treatment in posttraumatic period after experimental blood loss and combined traumatic brain injury. Bull Exp. Biol. Med., 164, 26-29. DOI: 10.1007/s10517-017-3918-4.
Lapshina, N.V., Lotina, A.S., Yablonskaya, Yu.A., Burnazyan, S.E., & Mullova, I.S. (2018). Cytoprotective therapy with mexicor in patients after radiofrequency ablation of atrial fibrillation to prevent early post-procedural arrhythmias. Anesthesiol. Bull., 91, 48-51 [in Russian].
Kesarev, O.G., Danilenko, L.M., Pokrovskii, M.V., Timokhina, A.S., & Khavanskii, A.V. (2017). Study of dose-dependent efect of 2-ethyl-6-methyl-3 hydroxypyridine succinate on the contractile function of isolated rat heat. Res. Result. Pharmacol. Clin. Pharmacol., 3, 3-9. DOI: 10.18413/ 2500-235X-2017-3-1-3-9.
Siegers, C.P., Voipel, V., Schel, G., & Younes, M. (1982). Effects of dithiocarb and (+)cyanidanol-3 on the CCl4 alcohol induced fibrosis of rat liver. Connective Tissue Norn. and Fibrotis. Hum. Liver, 3, 244-245. DOI: 10.1007/BF01965096.
Levchuk, R.D., Mikhailyuk, I.A., & Merlev, D.I. (2013). Pat. Ukraine for utility model No. 81107 IPC (2006.01) G 09 В 23/28. The method of polytrauma modeling. I. Horbachevsky Ternopil National Medical University inventors and assignee. No. u 2012 13575 stated. 27.11.2012; publ. 25.06.13, Bull. No. 12 [in Ukrainian].
Stefanov, O.V. (Ed.). (2001). Preclinical studies of drugs: guidelines. Kyiv: Avicenna. 528 p. [in Ukrainian].
Servatovych, A.M. (2015). Dynamics of disturbance processes of lipid peroxidation during the late manifestations of traumatic disease after cranio-skeletal injury and effectiveness of its correction by phytomixture. Bull. Sci. Res., 4, 106-109. DOI: 10.11603/1681-276Х.2015.4.5661 [in Ukrainian].
Prydruha, S.M. (2012). Dynamics of the content of lipid peroxidation products in the liver of rats during the late manifestations of traumatic disease and correction of deviations by thiotriazoline. Bull. Probl. Biol. Med., 1 (4), 244-247 [in Ukrainian].
Servatovych, А.М., & Gudyma, A.A. (2015). The role of antioxidant defense disturbances in pathogenesis of the late manifestations of traumatic disease after cranio-skeletal injury and effectiveness of its correction by phytomixture. J. Educ. Health Sport, 5 (12), 271-278. Retrieved from: http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5(12)%3A271-278.
Prydruha, S.M. (2013). Peculiarities of antioxidant protection in the pathogenesis of traumatic disease manifestations and their correction. Med. Chem., 15, 1 (54), 85-89 [in Ukrainian].
Jaeschke, H. (2011). Reactive oxygen and mechanism of inflammatory liver injury: Present concept. Gastorenterol. Hepatol., 26, l, 173-179.
Kozak, D.V. (2013). Antioxidant-prooxidant balance in liver tissue in the dynamics of the field of injury. Hospital Surg., 4 (64), 40-44 [in Ukrainian].
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